My Cart
My account
Menu
Main Menu

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ValidAb™

(HB9524)
Be the first to add a publication
Technical documents: SDS Datasheet
Be the first to review this product
Add to Wish List

Product overview

Name Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ValidAb™
Host Goat
Clonality Polyclonal
Target Rabbit IgG H&L
Conjugate Horseradish peroxidase (HRP)
Description

Goat Anti-Rabbit IgG H&L Horseradish peroxidase (HRP) secondary antibody. Part of the ValidAb™ range of highly validated, data-rich antibodies.

Tested applications Storage & Handling Product information Related Areas Technical guides (5) FAQs (5) Reviews References (3)
Write Your Own Review
You're reviewing:Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ValidAb™
Rate this item:

Validation data

Figure 1. Concentration response of HB8356 staining when using an anti-Calretinin (HB6494) primary antibody compared to Sigma A6154.
Figure 1. Concentration response of HB8356 staining when using an anti-Calretinin (HB6494) primary antibody compared to Sigma A6154.
Figure 2. No background staining observed when HB9524 is tested using a no-primary control.
Figure 2. No background staining observed when HB9524 is tested using a no-primary control.
Figure 3. HB9524 shows extremely high sensitivity producing bands down to a 1:2,000,000 dilution.
Figure 3. HB9524 shows extremely high sensitivity producing bands down to a 1:2,000,000 dilution.

Figure 1. Concentration response of HB8356 staining when using an anti-Calretinin (HB6494) primary antibody compared to Sigma A6154.

Figure 1. Concentration response of HB8356 staining when using an anti-Calretinin (HB6494) primary antibody compared to Sigma A6154.

Figure 2. No background staining observed when HB9524 is tested using a no-primary control.

Figure 2. No background staining observed when HB9524 is tested using a no-primary control.

Figure 3. HB9524 shows extremely high sensitivity producing bands down to a 1:2,000,000 dilution.

Figure 3. HB9524 shows extremely high sensitivity producing bands down to a 1:2,000,000 dilution.

Product information

Immunogen

Purified rabbit IgG

Isotype IgG
Purification notes

Immunogen affinity chromatography. Pre-adsorbed with mouse and human serum proteins

Concentration 0.35mg/ml
Formulation 50% Glycerol, 50% PBS pH 7.4 with 0.05% ProClin-300

Tested applications

Applications ELISA, IHC, WB
Western blot optimal concentration

Dependent on assay. A 1:20,000 dilution is a good starting point.

Open data link

Please follow this link to OSF

Storage & Handling

Storage instructions

-20°C

Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

FAQs

What guarantee do you have that my secondary antibody will perform as expected?

We guarantee that your secondary antibody will work for the applications we list on the datasheet. If the antibody fails to perform as expected then we are happy to offer a 100% refund guarantee. For more details please see our guarantee policy.

What protocols are available for use with this secondary antibody?

We have made a comprehensive collection of protocols that we have used in our experiments to validate this secondary antibody.

What counterstains do you recommend for use in ICC and IHC with this secondary antibody?

We recommend using either DAPI, Hoechst 33342 or Propidium Iodide to label cell nuclei. We also provide FITC Phalloidin and Rhodamine Phalloidin-TRITC for labelling actin filaments within cells.

Can I use sodium azide containing solutions to dilute this hrp conjugated antibody?

Sodium azide strongly inhibits the activity of horseradish peroxidase (HRP) therefore we do not recommend using sodium azide as a preservative in any diluent for this antibody.

Any other questions?

For any other questions about our antibody products please see our technical FAQs for antibodies

References for Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ValidAb™

References are publications that support the biological activity of the product

  • Coordinated interplay between palmitoylation, phosphorylation and SUMOylation regulates kainate receptor surface expression.

    Yucel BP et al (2023) Frontiers in molecular neuroscience 16 : 1270849
    Read more (PubMedID: 37868810)

  • The induction of p53 correlates with defects in the production, but not the levels, of the small ribosomal subunit and stalled large ribosomal subunit biogenesis.

    Eastham MJ et al (2023) Nucleic acids research 51 : 9397-9414
    Read more (PubMedID: 37526268)

  • Western blot: technique, theory, and trouble shooting.

    Mahmood T et al (2012) North American journal of medical sciences 4 : 429-34
    Read more (PubMedID: 23050259)
Related Products
Grouped product items
Order: HB9524
Pack PriceQty
1mL
$177

Goat Anti-Rabbit IgG H&L Horseradish peroxidase (HRP) secondary antibody. Part of the ValidAb™ range of highly validated, data-rich antibodies.

Shipping Info

Standard: $35

Hazardous: $35

Shipped on ice: $35

Shipping will be calculated at checkout

Contact Us

Call 609-683-7500
Fax 609-228-4994

customercare-usa@hellobio.com

New Products in this Area

Goat Anti-Mouse IgG H&L (Janelia Fluor® 549) preadsorbed ValidAb™
HB9240

Goat Anti-Mouse IgG H&L Janelia Fluor® 549 secondary antibody. Part of the ValidAb™ range of highly validated, data-rich antibodies.

Goat Anti-Mouse IgG H&L (Janelia Fluor® 525) preadsorbed ValidAb™
HB8144

Goat Anti-Mouse IgG H&L Janelia Fluor® 525 secondary antibody. Part of the ValidAb™ range of highly validated, data-rich antibodies.

Goat Anti-Rabbit IgG H&L (Janelia Fluor® 525) preadsorbed ValidAb™
HB6813

Goat Anti-Rabbit IgG H&L Janelia Fluor® 525 secondary antibody. Part of the ValidAb™ range of highly validated, data-rich antibodies.

Related Products

Goat Anti-Chicken IgY H&L (HRP) ValidAb™
Code:
HB9914
Pack:
1mL
Price:
$150

Goat Anti-Chicken IgG H&L Horseradish peroxidase (HRP) secondary antibody. Part of the ValidAb™ range of highly validated, data-rich antibodies.

SuperBlotTM ECL Western Blotting Substrate Kit (High sensitivity)
Code:
HB9308
Pack:
100mL, 250mL, 500mL
Price:
$170, $232, $425

High sensitivity ECL solution for developing chemiluminescent Western blots

Goat Anti-Mouse IgG H&L (HRP) preadsorbed ValidAb™
Code:
HB8356
Pack:
1mL
Price:
$177

Goat Anti-Mouse IgG H&L Horseradish peroxidase (HRP) secondary antibody. Part of the ValidAb™ range of highly validated, data-rich antibodies.