Fura-2 AM (Cell permeant)
(HB0780)
Product overview
| Name | Fura-2 AM (Cell permeant) |
| Biological description | Fura-2 AM (Cell permeant) is a high affinity, cell permeable calcium indicator which is ratiometric and UV light excitable. AM ester derivative of Fura-2.
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| Purity | >95% |
| Customer comments | Reliable product - product worked well for live cell calcium imaging in multiple cell types i.e. primary hippocampal neurons and HEK293 kidney cells. Verified customer, University College Dublin Reliable - I have tried Fura-2 AM across multiple cell types and in different assays. Works well and is reliable. Verified customer, UEA: University of East Anglia |
| Description | High affinity, cell permeable calcium indicator which is ratiometric and UV light excitable |
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Images
Figure 1. Fura-2 displays a shift in F340/F380 ratio in response to increased intracellular Ca2+.
500nM ionomycin (HB1002) was applied to HEK293 cells preincubated with Fura-2 AM after a baseline period. Ionomycin leads to increased intracellular Ca2+ therefore increases that 340nm signal associated with bound Ca2+ and decreases the 380nm associated with unbound Ca2+. This is displayed as a large increase in F340/F380 ratio which is a much more robust readout. For more details please see our Fura-2 protocol.
Figure 2. Ionomycin increases intracellular Ca2+ as measured by an increased F340/F380nm ratio.
500nM ionomycin (HB1002) was applied to HEK293 cells preincubated with Fura-2 AM leading to increased intracellular Ca2+ and therefore an increased F340/F380 ratio. For more details please see our Fura-2 protocol.
Figure 3. Carbachol stimulation of M3 receptors in HEK293 cells leads to Ca2+ release in a dose dependent manner.
Carbachol acts upon the Gq coupled muscarinic M3 receptors endogenously expressed in HEK293 cells to cause endoplasmic reticulum Ca2+ release which is detected using Fura-2 as a change in the ratio of fluorescence at 340 and 380nm. For more details please see our Fura-2 protocol.
Fura-2 AM (Cell permeant) product vial image | Hello Bio
Figure 1. Fura-2 displays a shift in F340/F380 ratio in response to increased intracellular Ca2+.
500nM ionomycin (HB1002) was applied to HEK293 cells preincubated with Fura-2 AM after a baseline period. Ionomycin leads to increased intracellular Ca2+ therefore increases that 340nm signal associated with bound Ca2+ and decreases the 380nm associated with unbound Ca2+. This is displayed as a large increase in F340/F380 ratio which is a much more robust readout. For more details please see our Fura-2 protocol.
Figure 2. Ionomycin increases intracellular Ca2+ as measured by an increased F340/F380nm ratio.
500nM ionomycin (HB1002) was applied to HEK293 cells preincubated with Fura-2 AM leading to increased intracellular Ca2+ and therefore an increased F340/F380 ratio. For more details please see our Fura-2 protocol.
Figure 3. Carbachol stimulation of M3 receptors in HEK293 cells leads to Ca2+ release in a dose dependent manner.
Carbachol acts upon the Gq coupled muscarinic M3 receptors endogenously expressed in HEK293 cells to cause endoplasmic reticulum Ca2+ release which is detected using Fura-2 as a change in the ratio of fluorescence at 340 and 380nm. For more details please see our Fura-2 protocol.
Fura-2 AM (Cell permeant) product vial image | Hello Bio
Biological Data
| Application notes | Please follow our Fura-2 AM protocol. |
Solubility & Handling
| Storage instructions | -20°C |
| Solubility overview | Soluble in DMSO |
| Handling | This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light. |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
| Purity | >95% |
| Chemical name | 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-amino-5'-methyl-phenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester |
| Molecular Weight | 1001.9 |
| Chemical structure | ![Fura-2 AM [108964-32-5] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb0780_2.png "Fura-2 AM [108964-32-5]") |
| Molecular Formula | C44H47N3O24 |
| CAS Number | 108964-32-5 |
| PubChem identifier | 3364574 |
| SMILES | CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=C3C(=C2)C=C(O3)C4=NC=C(O4)C(=O)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C |
| InChiKey | VPSRLGDRGCKUTK-UHFFFAOYSA-N |
| MDL number | MFCD00036976 |
| Appearance | Yellow solid |
| Excitation | 340/380nm |
| Emission | 505nm |
Technical guides
Technical Documentation
Fura-2 AM Protocol (231.3 KB) References for Fura-2 AM (Cell permeant)
References are publications that support the biological activity of the product
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Effects of transmitters and amyloid-beta peptide on calcium signals in rat cortical astrocytes: Fura-2AM measurements and stochastic model simulations.
Toivari E et al (2011) PLoS One 6(3) : e17914. -
Calcium imaging of cortical neurons using Fura-2 AM.
Barreto-Chang OL et al (2009) J Vis Exp -23 : -
Fura-2 measurement of cytosolic free Ca2+ in monolayers and suspensions of various types of animal cells.
Malgaroli A et al (1987) J Cell Biol 105(5) : 2145-55.
Publications
These publications cite the use of Fura-2 AM (Cell permeant) purchased from Hello Bio:
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Human Stem Cell-Derived TRPV1-Positive Sensory Neurons: A New Tool to Study Mechanisms of Sensitization.
Schrenk-Siemens K et al (2022) Cells 11PubMedID: 36139481 -
Insights into the Structure-Activity Relationship of Glycosides as Positive Allosteric Modulators Acting on P2X7 Receptors
Piyasirananda W et al (2021) Mol Pharmacol 99(2) : 163-174PubMedID: 33334897 -
Generation of the Human Pluripotent Stem-Cell-Derived Astrocyte Model with Forebrain Identity
Peteri UK et al (2021) Brain Sci 11(2)PubMedID: 33572154 -
Urokinase plasminogen activator mediates changes in human astrocytes modeling fragile X syndrome
Peteri UK et al (2021) Glia 69(12) : 2947-2962PubMedID: 34427356 -
PDX1 LOW MAFA LOW β-cells contribute to islet function and insulin release
Nasteska et al (2021) Nat Commun. 12(1) : 674PubMedID: 33514698
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