Fura-2 AM (Cell permeant)

(HB0780)
Technical documents: SDS CoA Datasheet

Product overview

Name Fura-2 AM (Cell permeant)
Description High affinity, cell permeable calcium indicator which is ratiometric and UV light excitable
Biological description

Fura-2 AM (Cell permeant) is a high affinity, cell permeable calcium indicator which is ratiometric and UV light excitable. AM ester derivative of Fura-2.


Fura-2 AM (Cell permeant) can noninvasively be loaded into live cells by incubation and is widely used for ratio-imaging microscopy and measuring intracellular calcium elevations in neurons and other excitable cells.


Excitation 340/380nm, Emission 505nm.

Purity >95%
Customer comments

Reliable product - product worked well for live cell calcium imaging in multiple cell types i.e. primary hippocampal neurons and HEK293 kidney cells. Verified customer, University College Dublin

Reliable - I have tried Fura-2 AM across multiple cell types and in different assays. Works well and is reliable. Verified customer, UEA: University of East Anglia

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Solubility & Handling

Storage instructions -20°C
Solubility overview Soluble in DMSO
Handling This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Chemical name 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-amino-5'-methyl-phenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester
Molecular Weight 1001.9
Chemical structure Product image
Molecular Formula C44H47N3O24
CAS Number 108964-32-5
PubChem identifier 3364574
SMILES CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=C3C(=C2)C=C(O3)C4=NC=C(O4)C(=O)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
InChi InChI=1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,
InChiKey VPSRLGDRGCKUTK-UHFFFAOYSA-N
MDL number MFCD00036976
Appearance Yellow solid

References for Fura-2 AM (Cell permeant)

References are publications that support the biological activity of the product
  • Calcium imaging of cortical neurons using Fura-2 AM.

    Barreto-Chang OL et al (2009) J Vis Exp -23 :
  • Effects of transmitters and amyloid-beta peptide on calcium signals in rat cortical astrocytes: Fura-2AM measurements and stochastic model simulations.

    Toivari E et al (2011) PLoS One 6(3) : e17914.
  • Fura-2 measurement of cytosolic free Ca2+ in monolayers and suspensions of various types of animal cells.

    Malgaroli A et al (1987) J Cell Biol 105(5) : 2145-55.
Publications
These publications cite the use of Fura-2 AM (Cell permeant) purchased from Hello Bio:
  • Ginsenosides act as positive modulators of P2X4 receptors.

    Dhuna et al (2018) Mol Pharmacol. pii: mol.118.113696. : doi: 10.1124/mol.118.113696.
    PubMedID: 30545933
  • Mapping a novel positive allosteric modulator binding site in the central vestibule region of human P2X7.

    Bidula et al (2019) Sci Rep. 9(1) : 3231
    PubMedID: 30824738
  • HESC-derived sensory neurons reveal an unexpected role for PIEZO2 in nociceptor mechanotransduction

    Schrenk-Siemens et al (2019) bioRxiv : https://doi.org/10.1101/741660
  • Mature and immature β-cells both contribute to islet function and insulin release

    Hodson et al (2020) Endocrinology & Metabolism Nature Research : Preprint
  • Vitamin D-binding protein is required for the maintenance of α-cell function and glucagon secretion

    Hodson et al (2019) bioRxiv This article is a preprint and has not been certified by peer review : https://doi.org/10.1101/2019.12.

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