Fura-2 AM (Cell permeant) is a high affinity, cell permeable calcium indicator which is ratiometric and UV light excitable. AM ester derivative of Fura-2.
Fura-2 AM (Cell permeant) can noninvasively be loaded into live cells by incubation and is widely used for ratio-imaging microscopy and measuring intracellular calcium elevations in neurons and other excitable cells.
Excitation 340/380nm, Emission 505nm.
Purity
>95%
Customer comments
Reliable product - product worked well for live cell calcium imaging in multiple cell types i.e. primary hippocampal neurons and HEK293 kidney cells. Verified customer, University College Dublin
Reliable - I have tried Fura-2 AM across multiple cell types and in different assays. Works well and is reliable. Verified customer, UEA: University of East Anglia
Description
High affinity, cell permeable calcium indicator which is ratiometric and UV light excitable
Figure 1. Fura-2 displays a shift in F340/F380 ratio in response to increased intracellular Ca2+.
500nM ionomycin (HB1002) was applied to HEK293 cells preincubated with Fura-2 AM after a baseline period. Ionomycin leads to increased intracellular Ca2+ therefore increases that 340nm signal associated with bound Ca2+ and decreases the 380nm associated with unbound Ca2+. This is displayed as a large increase in F340/F380 ratio which is a much more robust readout. For more details please see our Fura-2 protocol.
Figure 2. Ionomycin increases intracellular Ca2+ as measured by an increased F340/F380nm ratio.
500nM ionomycin (HB1002) was applied to HEK293 cells preincubated with Fura-2 AM leading to increased intracellular Ca2+ and therefore an increased F340/F380 ratio. For more details please see our Fura-2 protocol.
Figure 3. Carbachol stimulation of M3 receptors in HEK293 cells leads to Ca2+ release in a dose dependent manner.
Carbachol acts upon the Gq coupled muscarinic M3 receptors endogenously expressed in HEK293 cells to cause endoplasmic reticulum Ca2+ release which is detected using Fura-2 as a change in the ratio of fluorescence at 340 and 380nm. For more details please see our Fura-2 protocol.
Fura-2 AM (Cell permeant) product vial image | Hello Bio
Figure 1. Fura-2 displays a shift in F340/F380 ratio in response to increased intracellular Ca2+.
500nM ionomycin (HB1002) was applied to HEK293 cells preincubated with Fura-2 AM after a baseline period. Ionomycin leads to increased intracellular Ca2+ therefore increases that 340nm signal associated with bound Ca2+ and decreases the 380nm associated with unbound Ca2+. This is displayed as a large increase in F340/F380 ratio which is a much more robust readout. For more details please see our Fura-2 protocol.
Figure 2. Ionomycin increases intracellular Ca2+ as measured by an increased F340/F380nm ratio.
500nM ionomycin (HB1002) was applied to HEK293 cells preincubated with Fura-2 AM leading to increased intracellular Ca2+ and therefore an increased F340/F380 ratio. For more details please see our Fura-2 protocol.
Figure 3. Carbachol stimulation of M3 receptors in HEK293 cells leads to Ca2+ release in a dose dependent manner.
Carbachol acts upon the Gq coupled muscarinic M3 receptors endogenously expressed in HEK293 cells to cause endoplasmic reticulum Ca2+ release which is detected using Fura-2 as a change in the ratio of fluorescence at 340 and 380nm. For more details please see our Fura-2 protocol.
Fura-2 AM (Cell permeant) product vial image | Hello Bio
This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
References are publications that support the biological activity of the product
Effects of transmitters and amyloid-beta peptide on calcium signals in rat cortical astrocytes: Fura-2AM measurements and stochastic model simulations.
![Fura-2 AM [108964-32-5] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb0780_2.png "Fura-2 AM [108964-32-5]")
Fura-2 AM Protocol (231.3 KB) 
