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Fura-2 AM (Cell permeant)
(HB0780) Product overview
Name Fura-2 AM (Cell permeant)
Customer comments Reliable product - product worked well for live cell calcium imaging in multiple cell types i.e. primary hippocampal neurons and HEK293 kidney cells. Verified customer, University College Dublin
Reliable - I have tried Fura-2 AM across multiple cell types and in different assays. Works well and is reliable. Verified customer, UEA: University of East Anglia
Description High affinity, cell permeable calcium indicator which is ratiometric and UV light excitable Biological Data
Fura-2 AM (Cell permeant) is a high affinity, cell permeable calcium indicator which is ratiometric and UV light excitable. AM ester derivative of
Fura-2 AM (Cell permeant) can noninvasively be loaded into live cells by incubation and is widely used for ratio-imaging microscopy and measuring intracellular calcium elevations in neurons and other excitable cells.
Excitation 340/380nm, Emission 505nm. Solubility & Handling
Storage instructions -20°C
Solubility overview Soluble in DMSO
Handling This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. Chemical Data
Chemical name 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-amino-5'-methyl-phenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester
Molecular Formula C 44H 47N 3O 24
PubChem identifier 3364574
InChiKey VPSRLGDRGCKUTK-UHFFFAOYSA-N References for Fura-2 AM (Cell permeant)
References are publications that support the biological activity of the product
Effects of transmitters and amyloid-beta peptide on calcium signals in rat cortical astrocytes: Fura-2AM measurements and stochastic model simulations. Toivari E et al (2011) PLoS One 6(3) : e17914. Calcium imaging of cortical neurons using Fura-2 AM. Barreto-Chang OL et al (2009) J Vis Exp -23 : Fura-2 measurement of cytosolic free Ca2+ in monolayers and suspensions of various types of animal cells. Malgaroli A et al (1987) J Cell Biol 105(5) : 2145-55.
These publications cite the use of Fura-2 AM (Cell permeant) purchased from Hello Bio:
Human Stem Cell-Derived TRPV1-Positive Sensory Neurons: A New Tool to Study Mechanisms of Sensitization. Schrenk-Siemens K et al (2022) Cells 11 Insights into the Structure-Activity Relationship of Glycosides as Positive Allosteric Modulators Acting on P2X7 Receptors Piyasirananda W et al (2021) Mol Pharmacol 99(2) : 163-174 Generation of the Human Pluripotent Stem-Cell-Derived Astrocyte Model with Forebrain Identity Peteri UK et al (2021) Brain Sci 11(2) Urokinase plasminogen activator mediates changes in human astrocytes modeling fragile X syndrome Peteri UK et al (2021) Glia 69(12) : 2947-2962 PDX1 LOW MAFA LOW β-cells contribute to islet function and insulin release Nasteska et al (2021) Nat Commun. 12(1) : 674
High affinity, cell permeable calcium indicator which is ratiometric and UV light excitable