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Fura-2 AM (Cell permeant)

Name: Fura-2 AM (Cell permeant)
Brand: Hello Bio
SKU: HB0780
Price: 155 EUR
Availability: InStock
Rating: 100 (2 reviews)

(HB0780)

Publications (15)

Technical documents: SDS CoA Datasheet

Product overview

Name

Fura-2 AM (Cell permeant)

Purity

>95%

Customer comments

Reliable product - product worked well for live cell calcium imaging in multiple cell types i.e. primary hippocampal neurons and HEK293 kidney cells. Verified customer, University College Dublin

Reliable - I have tried Fura-2 AM across multiple cell types and in different assays. Works well and is reliable. Verified customer, UEA: University of East Anglia

Description

High affinity, cell permeable calcium indicator which is ratiometric and UV light excitable

Biological Data Solubility & Handling Chemical Data Related Areas Calculators Technical guides (1) Reviews (2) Publications (15) References (3) Frequently bought together

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Images

Fura-2 AM (Cell permeant) product vial image | Hello Bio

Biological Data

Biological description

Fura-2 AM (Cell permeant) is a high affinity, cell permeable calcium indicator which is ratiometric and UV light excitable. AM ester derivative of Fura-2.

Fura-2 AM (Cell permeant) can noninvasively be loaded into live cells by incubation and is widely used for ratio-imaging microscopy and measuring intracellular calcium elevations in neurons and other excitable cells.

Excitation 340/380nm, Emission 505nm.

Solubility & Handling

Storage instructions

-20°C

Solubility overview

Soluble in DMSO

Handling

This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.

Important

This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

More Info

Dilution

More Info

Chemical Data

Purity

>95%

Chemical name

1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-amino-5'-methyl-phenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester

Molecular Weight

1001.9

Chemical structure

Molecular Formula

C₄₄H₄₇N₃O₂₄

CAS Number

108964-32-5

PubChem identifier

3364574

SMILES

CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=C3C(=C2)C=C(O3)C4=NC=C(O4)C(=O)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C

InChi

InChI=1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,

InChiKey

VPSRLGDRGCKUTK-UHFFFAOYSA-N

MDL number

MFCD00036976

Appearance

Yellow solid

References for Fura-2 AM (Cell permeant)

References are publications that support the biological activity of the product

Effects of transmitters and amyloid-beta peptide on calcium signals in rat cortical astrocytes: Fura-2AM measurements and stochastic model simulations.
Toivari E et al (2011) PLoS One 6(3) : e17914.
Read more (PubMedID: 21483471)
Calcium imaging of cortical neurons using Fura-2 AM.
Barreto-Chang OL et al (2009) J Vis Exp -23 :
Read more (PubMedID: 19229178)
Fura-2 measurement of cytosolic free Ca2+ in monolayers and suspensions of various types of animal cells.
Malgaroli A et al (1987) J Cell Biol 105(5) : 2145-55.
Read more (PubMedID: 3680375)

Publications

Submit Yours Now

These publications cite the use of Fura-2 AM (Cell permeant) purchased from Hello Bio:

Human Stem Cell-Derived TRPV1-Positive Sensory Neurons: A New Tool to Study Mechanisms of Sensitization.
Schrenk-Siemens K et al (2022) Cells 11
PubMedID: 36139481
Insights into the Structure-Activity Relationship of Glycosides as Positive Allosteric Modulators Acting on P2X7 Receptors
Piyasirananda W et al (2021) Mol Pharmacol 99(2) : 163-174
PubMedID: 33334897
Generation of the Human Pluripotent Stem-Cell-Derived Astrocyte Model with Forebrain Identity
Peteri UK et al (2021) Brain Sci 11(2)
PubMedID: 33572154
Urokinase plasminogen activator mediates changes in human astrocytes modeling fragile X syndrome
Peteri UK et al (2021) Glia 69(12) : 2947-2962
PubMedID: 34427356
PDX1 LOW MAFA LOW β-cells contribute to islet function and insulin release
Nasteska et al (2021) Nat Commun. 12(1) : 674
PubMedID: 33514698

Technical guides

Understanding purity and quality - a guide for life scientists (159.4 KB)

High affinity, cell permeable calcium indicator which is ratiometric and UV light excitable

Order: HB0780
Pack	Price	Qty
1mg	€155

Fura-2 AM (Cell permeant)

Product overview

Images

Biological Data

Solubility & Handling

Calculators

Molarity

Dilution

Chemical Data

References for Fura-2 AM (Cell permeant)

Effects of transmitters and amyloid-beta peptide on calcium signals in rat cortical astrocytes: Fura-2AM measurements and stochastic model simulations.

Calcium imaging of cortical neurons using Fura-2 AM.

Fura-2 measurement of cytosolic free Ca2+ in monolayers and suspensions of various types of animal cells.

Human Stem Cell-Derived TRPV1-Positive Sensory Neurons: A New Tool to Study Mechanisms of Sensitization.

Insights into the Structure-Activity Relationship of Glycosides as Positive Allosteric Modulators Acting on P2X7 Receptors

Generation of the Human Pluripotent Stem-Cell-Derived Astrocyte Model with Forebrain Identity

Urokinase plasminogen activator mediates changes in human astrocytes modeling fragile X syndrome

PDX1 LOW MAFA LOW β-cells contribute to islet function and insulin release

Technical guides

About you

Tell us about your publication!

Fura-2 AM (Cell permeant)

Product overview

Images

Biological Data

Solubility & Handling

Calculators

Molarity

Dilution

Chemical Data

References for Fura-2 AM (Cell permeant)

Effects of transmitters and amyloid-beta peptide on calcium signals in rat cortical astrocytes: Fura-2AM measurements and stochastic model simulations.

Calcium imaging of cortical neurons using Fura-2 AM.

Fura-2 measurement of cytosolic free Ca2+ in monolayers and suspensions of various types of animal cells.

Human Stem Cell-Derived TRPV1-Positive Sensory Neurons: A New Tool to Study Mechanisms of Sensitization.

Insights into the Structure-Activity Relationship of Glycosides as Positive Allosteric Modulators Acting on P2X7 Receptors

Generation of the Human Pluripotent Stem-Cell-Derived Astrocyte Model with Forebrain Identity

Urokinase plasminogen activator mediates changes in human astrocytes modeling fragile X syndrome

PDX1 LOW MAFA LOW β-cells contribute to islet function and insulin release

Technical guides

About you

Tell us about your publication!

About you

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