Product overview
| Name | Fura-2 AM (Cell permeant) |
| Description | High affinity, cell permeable calcium indicator which is ratiometric and UV light excitable |
| Purity | >95% |
| Customer comments | Reliable product - product worked well for live cell calcium imaging in multiple cell types i.e. primary hippocampal neurons and HEK293 kidney cells. Verified customer, University College Dublin Reliable - I have tried Fura-2 AM across multiple cell types and in different assays. Works well and is reliable. Verified customer, UEA: University of East Anglia |
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Biological Data
| Biological description | Fura-2 AM (Cell permeant) is a high affinity, cell permeable calcium indicator which is ratiometric and UV light excitable. AM ester derivative of Fura-2.
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Solubility & Handling
| Storage instructions | -20°C |
| Solubility overview | Soluble in DMSO |
| Handling | This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light. |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
| Chemical name | 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-amino-5'-methyl-phenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester |
| Molecular Weight | 1001.9 |
| Chemical structure |  |
| Molecular Formula | C44H47N3O24 |
| CAS Number | 108964-32-5 |
| PubChem identifier | 3364574 |
| SMILES | CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=C3C(=C2)C=C(O3)C4=NC=C(O4)C(=O)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C |
| InChi | InChI=1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10, |
| InChiKey | VPSRLGDRGCKUTK-UHFFFAOYSA-N |
| MDL number | MFCD00036976 |
| Appearance | Yellow solid |
References for Fura-2 AM (Cell permeant)
References are publications that support the biological activity of the product
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Effects of transmitters and amyloid-beta peptide on calcium signals in rat cortical astrocytes: Fura-2AM measurements and stochastic model simulations.
Toivari E et al (2011) PLoS One 6(3) : e17914. -
Calcium imaging of cortical neurons using Fura-2 AM.
Barreto-Chang OL et al (2009) J Vis Exp -23 : -
Fura-2 measurement of cytosolic free Ca2+ in monolayers and suspensions of various types of animal cells.
Malgaroli A et al (1987) J Cell Biol 105(5) : 2145-55.
Publications
These publications cite the use of Fura-2 AM (Cell permeant) purchased from Hello Bio:
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PDX1 LOW MAFA LOW β-cells contribute to islet function and insulin release
Nasteska et al (2021) Nat Commun. 12(1) : 674PubMedID: 33514698 -
Mature and immature β-cells both contribute to islet function and insulin release
Hodson et al (2020) Endocrinology & Metabolism Nature Research : PreprintPubMedID: DOI: 10.21203/rs.3.rs-40718/v1 -
CXCL14 Preferentially Synergizes With Homeostatic Chemokine Receptor Systems
Kouzeli et al (2020) Front Immunol. 5;11 : 561404PubMedID: 33123134 -
Mapping a novel positive allosteric modulator binding site in the central vestibule region of human P2X7.
Bidula et al (2019) Sci Rep. 9(1) : 3231PubMedID: 30824738 -
HESC-derived sensory neurons reveal an unexpected role for PIEZO2 in nociceptor mechanotransduction
Schrenk-Siemens et al (2019) bioRxiv : https://doi.org/10.1101/741660
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