Fura-2 AM (Cell permeant)

(HB0780)
Technical documents: SDS CoA Datasheet

Product overview

Name Fura-2 AM (Cell permeant)
Description High affinity, cell permeable calcium indicator which is ratiometric and UV light excitable
Purity >95%
Customer comments

Reliable product - product worked well for live cell calcium imaging in multiple cell types i.e. primary hippocampal neurons and HEK293 kidney cells. Verified customer, University College Dublin

Reliable - I have tried Fura-2 AM across multiple cell types and in different assays. Works well and is reliable. Verified customer, UEA: University of East Anglia

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Biological Data

Biological description

Fura-2 AM (Cell permeant) is a high affinity, cell permeable calcium indicator which is ratiometric and UV light excitable. AM ester derivative of Fura-2.


Fura-2 AM (Cell permeant) can noninvasively be loaded into live cells by incubation and is widely used for ratio-imaging microscopy and measuring intracellular calcium elevations in neurons and other excitable cells.


Excitation 340/380nm, Emission 505nm.

Solubility & Handling

Storage instructions -20°C
Solubility overview Soluble in DMSO
Handling This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Chemical name 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-amino-5'-methyl-phenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester
Molecular Weight 1001.9
Chemical structure Fura-2 AM  [108964-32-5] Chemical Structure
Molecular Formula C44H47N3O24
CAS Number 108964-32-5
PubChem identifier 3364574
SMILES CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=C3C(=C2)C=C(O3)C4=NC=C(O4)C(=O)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
InChi InChI=1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,
InChiKey VPSRLGDRGCKUTK-UHFFFAOYSA-N
MDL number MFCD00036976
Appearance Yellow solid

References for Fura-2 AM (Cell permeant)

References are publications that support the biological activity of the product
  • Effects of transmitters and amyloid-beta peptide on calcium signals in rat cortical astrocytes: Fura-2AM measurements and stochastic model simulations.

    Toivari E et al (2011) PLoS One 6(3) : e17914.
  • Calcium imaging of cortical neurons using Fura-2 AM.

    Barreto-Chang OL et al (2009) J Vis Exp -23 :
  • Fura-2 measurement of cytosolic free Ca2+ in monolayers and suspensions of various types of animal cells.

    Malgaroli A et al (1987) J Cell Biol 105(5) : 2145-55.
Publications
These publications cite the use of Fura-2 AM (Cell permeant) purchased from Hello Bio:
  • Insights into the Structure-Activity Relationship of Glycosides as Positive Allosteric Modulators Acting on P2X7 Receptors

    Piyasirananda W et al (2021) Mol Pharmacol 99(2) : 163-174
    PubMedID: 33334897
  • Generation of the Human Pluripotent Stem-Cell-Derived Astrocyte Model with Forebrain Identity

    Peteri UK et al (2021) Brain Sci 11(2)
    PubMedID: 33572154
  • Urokinase plasminogen activator mediates changes in human astrocytes modeling fragile X syndrome

    Peteri UK et al (2021) Glia 69(12) : 2947-2962
    PubMedID: 34427356
  • PDX1 LOW MAFA LOW β-cells contribute to islet function and insulin release

    Nasteska et al (2021) Nat Commun. 12(1) : 674
    PubMedID: 33514698
  • Revisiting the Idea That Amyloid-β Peptide Acts as an Agonist for P2X7

    Bibic et al (2020) Front Mol Neurosci 13 : 166
    PubMedID: 33071753

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