Figure 1. BrdU immunoreactivity in serum starved and subsequently stimulated HEK293T cells
BrdU is an exogenous DNA base that intercalates into DNA during replication. BrdU from Hello Bio was used at 10 µM alongside an anti-BrdU monoclonal antibody (HB9919) to demonstrate the lack of proliferation under serum starved conditions which reverses upon incubation with 20% FBS. For more details see #Protocol 1 in application notes below.
Figure 2. BrdU immunoreactivity in cultured HEK293T cells
BrdU is an exogenous DNA base that intercalates into DNA during replication. BrdU from Hello Bio was used at 10 µM to label proliferating cells which was then detected using an anti-BrdU monoclonal antibody (HB9919) at 2 µg/ml to reveal proliferating cells. For more details see #Protocol 2 in application notes below.
BrdU (5-Bromo-2′-deoxyuridine) product vial image | Hello Bio
Figure 1. BrdU immunoreactivity in serum starved and subsequently stimulated HEK293T cells
BrdU is an exogenous DNA base that intercalates into DNA during replication. BrdU from Hello Bio was used at 10 µM alongside an anti-BrdU monoclonal antibody (HB9919) to demonstrate the lack of proliferation under serum starved conditions which reverses upon incubation with 20% FBS. For more details see #Protocol 1 in application notes below.
Figure 2. BrdU immunoreactivity in cultured HEK293T cells
BrdU is an exogenous DNA base that intercalates into DNA during replication. BrdU from Hello Bio was used at 10 µM to label proliferating cells which was then detected using an anti-BrdU monoclonal antibody (HB9919) at 2 µg/ml to reveal proliferating cells. For more details see #Protocol 2 in application notes below.
BrdU (5-Bromo-2′-deoxyuridine) product vial image | Hello Bio
Biological Data
Biological description
Thymidine analog which is incorporated into DNA during DNA replication (during S-phase of cell cycle).
BrdU is used to identify proliferating cells. Labels cell lines and primary cell cultures in vitro and also cells in vivo. Suitable for in vivo use.
Widely used to study adult neurogenesis. Can be used in combination with neuron specific markers such as NeuN to identify newly formed neurons.
Frequently used to label and fate-map dividing cells in neural stem cell biology.
Application notes
#Protocol 1: Serum starved vs stimulated BrdU proliferation assay
HEK293T cells were cultured until approximately 50% confluency in 10% FBS in DMEM before media was exchanged to pure DMEM for 24hrs.
Cells were either left in DMEM (serum starved) or incubated in 20% FBS in DMEM (serum starved + stimulated) for 2hrs in the prescence of 10µM BrdU (HB0978).
Cells were fixed in 4% PFA and then stained for BrdU using a monoclonal anti-BrdU antibody (HB9919) following our ICC protocol then imaged using a confocal microscope.
#Protocol 2: Proliferation of HEK293T cells visualised using BrdU
BrdU cells were cultured in 10% FBS in DMEM in the prescence of 10µM BrdU (HB0978) for 5 days.
Cells were fixed in 4% PFA and then stained for BrdU using a monoclonal anti-BrdU antibody (HB9919) following our ICC protocol then imaged using a confocal microscope.
Solubility & Handling
Storage instructions
-20°C
Solubility overview
Soluble in water (50mM) and in DMSO (100mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
Y-27632 is a selective ROCK inhibitor. Frequently used 3D growth matrix component and for production of organoids. Also used when inducing neurons from fibroblasts. Manufactured to a high degree of optical purity.
![BrdU [59-14-3] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb0979.png "BrdU [59-14-3]")
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