BrdU (5-Bromo-2′-deoxyuridine)
(HB0979)
Product overview
Name | BrdU (5-Bromo-2′-deoxyuridine) |
Description | Thymidine analog. Widely used to identify proliferating cells, enhances Yamanaka factor reprogramming |
Alternative names | 5-BrdU, 5-bromo-2'-deoxyuridine, Broxuridine |
Purity | >98% |
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Biological Data
Biological description | Thymidine analog which is incorporated into DNA during DNA replication (during S-phase of cell cycle).
Frequently used to label and fate-map dividing cells in neural stem cell biology. |
Application notes | #Protocol 1: Serum starved vs stimulated BrdU proliferation assay
#Protocol 2: Proliferation of HEK293T cells visualised using BrdU
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Solubility & Handling
Storage instructions | -20°C |
Solubility overview | Soluble in water (50mM) and in DMSO (100mM) |
Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
Purity | >98% |
Chemical name | 5-Bromo-2-deoxyuridine |
Molecular Weight | 307.1 |
Chemical structure | |
Molecular Formula | C9H11BrN2O5 |
CAS Number | 59-14-3 |
PubChem identifier | 6035 |
SMILES | C1[C@@H]([C@H](O[C@H]1N2C=C(C(=O)NC2=O)Br)CO)O |
Source | Synthetic |
InChi | InChI=1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1 |
InChiKey | WOVKYSAHUYNSMH-RRKCRQDMSA-N |
MDL number | MFCD00006529 |
Appearance | White solid |
References for BrdU (5-Bromo-2′-deoxyuridine)
References are publications that support the biological activity of the product
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The dark side of BrdU in neural stem cell biology: detrimental effects on cell cycle, differentiation and survival.
Lehner B et al (2011) Cell Tissue Res 345(3) : 313-28. -
BrdU assay for neurogenesis in rodents.
Wojtowicz JM et al (2006) Nature protocols 1 : 1399-405 -
Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus.
van Praag H et al (1999) Nature neuroscience 2 : 266-70 -
Neurogenesis in the adult human hippocampus.
Eriksson PS et al (1998) Nat Med 4(11) : 1313-7. -
Pharmacological evaluation of intravenous delivery of 5-bromodeoxyuridine to patients with brain tumors.
Russo A et al (1984) Cancer Res 44(4) : 1702-5.