|Name|| BrdU (5-Bromo-2′-deoxyuridine) |
|Description|| Thymidine analog. Widely used to identify proliferating cells, enhances Yamanaka factor reprogramming |
|Alternative names|| 5-BrdU, 5-bromo-2'-deoxyuridine, Broxuridine |
|Biological description|| |
Thymidine analog which is incorporated into DNA during DNA replication (during S-phase of cell cycle).
BrdU is used to identify proliferating cells. Labels cell lines and primary cell cultures in vitro and also cells in vivo.
Widely used to study adult neurogenesis. Can be used in combination with neuron specific markers such as NeuN to identify newly formed neurons.
Frequently used to label and fate-map dividing cells in neural stem cell biology.
Solubility & Handling
|Storage instructions|| -20°C |
|Solubility overview|| Soluble in water (50mM) and in DMSO (100mM) |
|Important|| This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
|Chemical name|| 5-Bromo-2-deoxyuridine |
|Chemical structure|| |
|Molecular Formula|| C9H11BrN2O5 |
|SMILES|| C1[C@@H]([C@H](O[C@H]1N2C=C(C(=O)NC2=O)Br)CO)O |
|InChi|| InChI=1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1 |
|InChiKey|| WOVKYSAHUYNSMH-RRKCRQDMSA-N |
References for BrdU (5-Bromo-2′-deoxyuridine)
References are publications that support the biological activity of the product
The dark side of BrdU in neural stem cell biology: detrimental effects on cell cycle, differentiation and survival.
Lehner B et al (2011) Cell Tissue Res 345(3) : 313-28.
Neurogenesis in the adult human hippocampus.
Eriksson PS et al (1998) Nat Med 4(11) : 1313-7.
Pharmacological evaluation of intravenous delivery of 5-bromodeoxyuridine to patients with brain tumors.
Russo A et al (1984) Cancer Res 44(4) : 1702-5.