Product overview
| Name | SuperBlotTM Rapid Single-Step Blocking Solution |
| Biological description | SuperBlot™ Rapid Single-Step Blocking Solution is a novel single-step blocking solution for Western Blot that enables the rapid blocking and staining of membranes in a single stage:
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| Description | Rapid single-step blocking solution and signal enhancer for Western Blotting |
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Images
Figure 1. Speedy GAPDH staining in rat brain samples using SuperBlot™ Rapid Single-Step Blocking Solution
Rat brain cytosol fractions were loaded (see Molnar et al., 1993. Neuroscience 53:307-326) at 20µg / lane onto a 4-20% tris-glycine acrylamide gel alongside a protein ladder before being run at 160V for 60 minutes. Wet transfer to a PVDF membrane was completed in 90 minutes using 400mA. The membrane was then incubated simultaneously in SuperBlot™ Rapid Single-Step Blocking Solution, HB9177 (mouse monoclonal anti-GAPDH, 1:4,000, 0.25μg/ml) and HB8356 (goat polyclonal anti-mouse HRP, 1:20,000) for 60 minutes. Following washing, the membrane was developed using a ECL substrate kit and a Licor Odyssey Fc imaging system (ECL channel: 5 min exposure, 700nm channel: 30 sec exposure). For more detail please see our Western blotting protocol.
Figure 2. SuperBlot™ Rapid Single-Step Blocking Solution enables signal detection in as little as 15 minutes.
Rat brain cytosol fractions were loaded (see Molnar et al., 1993. Neuroscience 53:307-326) at 20µg / lane onto a 4-20% tris-glycine acrylamide gel alongside a protein ladder before being run at 160V for 60 minutes. Wet transfer to a PVDF membrane was completed in 90 minutes using 400mA. The membrane was then cut into strips and incubated for either 15, 30, 60 or 120 minutes in SuperBlot™ Rapid Single-Step Blocking Solution, HB9177 (mouse monoclonal anti-GAPDH, 1:4,000, 0.25μg/ml) and HB8356 (goat polyclonal anti-mouse HRP, 1:20,000). After the incubation each strip was placed in wash buffer and then subsequently washed together so that they could be imaged at the same time. The strips were developed using a ECL substrate kit and a Licor Odyssey Fc imaging system (ECL channel: 5 min exposure, 700nm channel: 30 sec exposure). For more detail please see our Western blotting protocol.
Figure 1. Speedy GAPDH staining in rat brain samples using SuperBlot™ Rapid Single-Step Blocking Solution
Rat brain cytosol fractions were loaded (see Molnar et al., 1993. Neuroscience 53:307-326) at 20µg / lane onto a 4-20% tris-glycine acrylamide gel alongside a protein ladder before being run at 160V for 60 minutes. Wet transfer to a PVDF membrane was completed in 90 minutes using 400mA. The membrane was then incubated simultaneously in SuperBlot™ Rapid Single-Step Blocking Solution, HB9177 (mouse monoclonal anti-GAPDH, 1:4,000, 0.25μg/ml) and HB8356 (goat polyclonal anti-mouse HRP, 1:20,000) for 60 minutes. Following washing, the membrane was developed using a ECL substrate kit and a Licor Odyssey Fc imaging system (ECL channel: 5 min exposure, 700nm channel: 30 sec exposure). For more detail please see our Western blotting protocol.
Figure 2. SuperBlot™ Rapid Single-Step Blocking Solution enables signal detection in as little as 15 minutes.
Rat brain cytosol fractions were loaded (see Molnar et al., 1993. Neuroscience 53:307-326) at 20µg / lane onto a 4-20% tris-glycine acrylamide gel alongside a protein ladder before being run at 160V for 60 minutes. Wet transfer to a PVDF membrane was completed in 90 minutes using 400mA. The membrane was then cut into strips and incubated for either 15, 30, 60 or 120 minutes in SuperBlot™ Rapid Single-Step Blocking Solution, HB9177 (mouse monoclonal anti-GAPDH, 1:4,000, 0.25μg/ml) and HB8356 (goat polyclonal anti-mouse HRP, 1:20,000). After the incubation each strip was placed in wash buffer and then subsequently washed together so that they could be imaged at the same time. The strips were developed using a ECL substrate kit and a Licor Odyssey Fc imaging system (ECL channel: 5 min exposure, 700nm channel: 30 sec exposure). For more detail please see our Western blotting protocol.
Biological Data
| Application notes | One-Step Blocking Protocol
For more information about Western Blotting including buffer recipes please see our Western Blot Protocol. It is possible to store and re-use the mixed antibodies in blocking solution at 4°C for up to a month.
Two-Step Blocking Protocol
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Solubility & Handling
| Storage instructions | Room temperature |
| Storage buffer | Contains PBS amongst other constituents |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
Technical guides
Western Blot Protocol (1 MB) References for SuperBlotTM Rapid Single-Step Blocking Solution
References are publications that support the biological activity of the product
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An overview of technical considerations for Western blotting applications to physiological research.
Bass JJ et al (2017) Scandinavian journal of medicine & science in sports 27 : 4-25 -
Western blot: technique, theory, and trouble shooting.
Mahmood T et al (2012) North American journal of medical sciences 4 : 429-34
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