Product overview
| Name | Mini BCA Protein Assay Kit |
| Biological description | Simple, rapid, detergent tolerant (up to 5%) kit for determining the concentration of proteins in solution. This kit is optimized to measure protein concentrations from 0.5 to 200 μg/mL.
Key features of the mini-BCA Protein Assay Kit:
|
| Description | Simple, rapid, detergent tolerant kit for measuring the concentration of proteins in solution (0.5 to 200 μg/mL). |
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Images
Figure 1. Standard curve of recombinant albumin standards and gamma globulins using Hello Bio Mini BCA Protein Assay Kit
Using the Hello Bio Mini BCA Protein Assay Kit with the supplied standards produces a accurate standard curve (R2=0.99) that can be used to calculate the protein concentration of unknown standards. Additionally the kit was used to construct a standard curve of gamma-globulins (R2=0.98) to show that the assay is generalizable across proteins. Standards (n=4) are shown ± standard deviation.
Figure 2. The Mini BCA Protein Assay is a colorimetric assay developing a purple color correlating with protein concentration.
The Mini BCA assay produces a deep purple color that correlates with the concentration of protein in a sample. The assay is based upon the ability of peptide bonds in a protein to reduce Cu2+ ions to Cu1+ in a concentration dependent manner. The Cu1+ ions then are chelated by bicinchoninic acid to form a strongly purple colored solution that maximally absorbs at 562nm. To enhance sensitivity over the standard BCA assay the mini BCA assay uses more concentrated reagents in 3 solutions.
Figure 1. Standard curve of recombinant albumin standards and gamma globulins using Hello Bio Mini BCA Protein Assay Kit
Using the Hello Bio Mini BCA Protein Assay Kit with the supplied standards produces a accurate standard curve (R2=0.99) that can be used to calculate the protein concentration of unknown standards. Additionally the kit was used to construct a standard curve of gamma-globulins (R2=0.98) to show that the assay is generalizable across proteins. Standards (n=4) are shown ± standard deviation.
Figure 2. The Mini BCA Protein Assay is a colorimetric assay developing a purple color correlating with protein concentration.
The Mini BCA assay produces a deep purple color that correlates with the concentration of protein in a sample. The assay is based upon the ability of peptide bonds in a protein to reduce Cu2+ ions to Cu1+ in a concentration dependent manner. The Cu1+ ions then are chelated by bicinchoninic acid to form a strongly purple colored solution that maximally absorbs at 562nm. To enhance sensitivity over the standard BCA assay the mini BCA assay uses more concentrated reagents in 3 solutions.
Biological Data
| Application notes | Mini BCA Assay Protocol
Before using the kit for the first time add 5ml of buffer to each tube of lyophilised standard. For best accuracy use the same buffer as your proteins of interest. The assay can either be carried out in microplate or tube format.
Microplate Protocol
Tube Protocol
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Solubility & Handling
| Storage instructions | Room temperature |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
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