Figure 1. Visualize proteins in as little as 15 minutes with SuperBlotTM Rapid Coomassie Staining Solution
A 4-20% Tris-Glycine gel was loaded with 20µg/lane of a rat brain cytosol preparation (see Molnar et al., 1993. Neuroscience 53:307-326) and run for approximately 60 minutes at 160V. The gel was subsequently incubated in Rapid Coomassie for 15 minutes, briefly rinsed in dH2O then imaged.
Figure 2. High sensitivity staining in 60 minutes with Rapid Coomassie
A 4-20% Tris-Glycine gel was loaded with varying amounts of a rat brain cytosol preparation (0.31 - 20µg / lane; see Molnar et al., 1993. Neuroscience 53:307-326) and run for approximately 60 minutes at 160V. The gel was subsequently incubated in Rapid Coomassie for 60 minutes, briefly rinsed in multiple changes of dH2O then imaged.
Figure 1. Visualize proteins in as little as 15 minutes with SuperBlotTM Rapid Coomassie Staining Solution
A 4-20% Tris-Glycine gel was loaded with 20µg/lane of a rat brain cytosol preparation (see Molnar et al., 1993. Neuroscience 53:307-326) and run for approximately 60 minutes at 160V. The gel was subsequently incubated in Rapid Coomassie for 15 minutes, briefly rinsed in dH2O then imaged.
Figure 2. High sensitivity staining in 60 minutes with Rapid Coomassie
A 4-20% Tris-Glycine gel was loaded with varying amounts of a rat brain cytosol preparation (0.31 - 20µg / lane; see Molnar et al., 1993. Neuroscience 53:307-326) and run for approximately 60 minutes at 160V. The gel was subsequently incubated in Rapid Coomassie for 60 minutes, briefly rinsed in multiple changes of dH2O then imaged.
Biological Data
Application notes
Once the gel has finished running remove it from the cassette and place immediately in ≈20ml of rapid Coomassie stain. Incubate for at least 15 minutes then pour off the stain. Briefly rinse with dH2O then image.
A 15 minute incubation is sufficient for most protein visualization however for increased sensitivity then the incubation time can be increased. If increasing the incubation time then increased washing until the optimal signal/noise ratio is achieved is recommended.
Solubility & Handling
Storage instructions
Room temperature
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use