SuperBlotTM Rapid Coomassie Staining Solution

(HB7028)
Technical documents: Datasheet

Product overview

Name SuperBlotTM Rapid Coomassie Staining Solution
Biological description
  • Unique nanoparticle Coomassie formulation enabling rapid staining of proteins in polyacrylamide gels in as little as 15 minutes.
  • Requires minimal washing to achieve low background.
  • Safer and more environmentally friendly than traditional alternatives.
  • Compatible with subsequent mass spectrometry analysis and the acetic acid free formulation means proteins are not methylated or acetylated.
Description

Rapid coomassie protein staining solution

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Images

Figure 1. Visualize proteins in as little as 15 minutes with SuperBlotTM Rapid Coomassie Staining Solution

A 4-20% Tris-Glycine gel was loaded with 20µg/lane of a rat brain cytosol preparation (see Molnar et al., 1993. Neuroscience 53:307-326) and run for approximately 60 minutes at 160V. The gel was subsequently incubated in Rapid Coomassie for 15 minutes, briefly rinsed in dH2O then imaged.

Figure 2. High sensitivity staining in 60 minutes with Rapid Coomassie

A 4-20% Tris-Glycine gel was loaded with varying amounts of a rat brain cytosol preparation (0.31 - 20µg / lane; see Molnar et al., 1993. Neuroscience 53:307-326) and run for approximately 60 minutes at 160V. The gel was subsequently incubated in Rapid Coomassie for 60 minutes, briefly rinsed in multiple changes of dH2O then imaged.

Biological Data

Application notes

Once the gel has finished running remove it from the cassette and place immediately in ≈20ml of rapid Coomassie stain. Incubate for at least 15 minutes then pour off the stain. Briefly rinse with dH2O then image.

 

A 15 minute incubation is sufficient for most protein visualization however for increased sensitivity then the incubation time can be increased. If increasing the incubation time then increased washing until the optimal signal/noise ratio is achieved is recommended.

Solubility & Handling

Storage instructions

Room temperature

Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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References for SuperBlotTM Rapid Coomassie Staining Solution

References are publications that support the biological activity of the product

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