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SB 216763

Technical documents: SDS CoA Datasheet

Product overview

Name SB 216763
Alternative names SB-216763
Purity >98%
Description Potent, selective cell permeable GSK-3 inhibitor. Maintains mESCs and promotes retinal stem cells proliferation.
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Biological Data

Biological description

Potent, selective, cell permeable and ATP competitive GSK-3 inhibitor which is equally effective at GSK-3α as GSK-3β (IC50 = 34 nM for GSK-3α). Displays little activity at 24 other serine/threonine and tyrosine protein kinases.

SB 216763 is active in vivo.

The compound stimulates glycogen synthesis (EC50 = 3.6 µM), acts as a neuroprotectant and prevents neuronal cell death. It also shows anti-inflammatory and cardioprotective actions. 

It is also widely used in stem cell research, for example:


  • Maintains mESCs (mouse embryonic stem cells) in a pluripotent state in the absence of LIF when cultured with MEFs
  • Restores INS-dependent differentiation of C2ind myoblasts


  • Promotes the conversion of human umbilical cord mesenchymal stem cells into neural precursors
  • Enhances chondrogenic differentiation of hWJ-MSCs
  • Increases neurogenesis of human neural progenitor cell differentiation
  • Promotes neural differentiation of CD117-positive hAFS cells towards neural progenitor cells


  • Promotes the proliferation of retinal stem cells

Solubility & Handling

Storage instructions Room temperature
Solubility overview Soluble in DMSO (75mM)
Handling This compound is light sensitive; we therefore recommend protecting the solid material and solutions from exposure to light.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.



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Chemical Data

Purity >98%
Chemical name 3-(2,4-Dichlorophenyl)-4-(1-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione
Molecular Weight 371.22
Molecular Formula C19H12Cl2N2O2
CAS Number 280744-09-4
PubChem identifier 176158
SMILES CN1C=C(C2=CC=CC=C21)C3=C(C(=O)NC3=O)C4=C(C=C(C=C4)Cl)Cl
Source Synthetic
InChi InChI=1S/C19H12Cl2N2O2/c1-23-9-13(11-4-2-3-5-15(11)23)17-16(18(24)22-19(17)25)12-7-6-10(20)8-14(12)21/h2-9H,1H3,(H,22,24,25)
MDL number MFCD09753369
Appearance Orange solid

References for SB 216763

References are publications that support the biological activity of the product
  • Glycogen synthase kinase 3 (GSK3)-inhibitor SB216763 promotes the conversion of human umbilical cord mesenchymal stem cells into neural precursors in adherent culture.

    Gao et al (2017) Hum Cell 30(1) : 11-22
  • Stemistry: the control of stem cells in situ using chemistry.

    Davies et al (2015) J Med Chem 58(7) : 2863-94
  • Glycogen synthase kinase 3 (GSK3) inhibitor, SB-216763, promotes pluripotency in mouse embryonic stem cells.

    Kirby et al (2012) PLoS One 6 : e39329
  • Small molecule GSK-3 inhibitors increase neurogenesis of human neural progenitor cells.

    Lange et al (2011) Neurosci Lett. 488(1) : 36-40
  • 3-(2,4-dichlorophenyl)-4-(1-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione (SB216763), a glycogen synthase kinase-3 inhibitor, displays therapeutic properties in a mouse model of pulmonary inflammation and fibrosis.

    Gurrieri C et al (2010) J Pharmacol Exp Ther 332(3) : 785-94.

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