ED50 = 15-25µg/ml (determined by its ability to stimulate of proliferation of rat C6 cells) corresponding to a specific activity of 40-67units/mg.
Solubility & Handling
Solubility overview
To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 100μg/ml, which can then be diluted to make a working solution
Handling
Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
Transport of human recombinant brain-derived neurotrophic factor (BDNF) through the rat blood-brain barrier in vivo using vector-mediated peptide drug delivery
Pardridge WM et al (1994) Pharm Res 11(5) : 738-46
Understanding purity and quality - a guide for life scientists
