Product overview

Name Rapamycin
Description mTOR inhibitor and potent immunosuppressant. Also used in inducible gene editing methods (e.g. CRISPR/Cas9) and promotes hPSC differentiation.
Alternative names Sirolimus
Purity >98%
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Biological Data

Biological description

Overview

Rapamycin is an mTOR inhibitor and potent immunosuppressant. It complexes with FKBP12 to bind mTORC1 to inhibit mTOR activity and block subsequent activation of p70 s6 kinase (IC50 = 50 pM).

Active in vivo.

Uses and applications

Rapamcyin shows a variety of bioloigical actions. E.g it induces autophagy and apoptosis.

Promotes hPSC differentiation to blood progenitor and mesendoderm cells.

It can also be used in inducible CRISPR/Cas9 systems to enable inducible gene editing.

Used as a chemical dimerizer in Chemically-inducible dimerization (CID).

As mTOR is a central inflammation regulator, rapamycin has recently been investigated as part of cytokine storm / COVID-19 related research.

Solubility & Handling

Storage instructions -20°C
Solubility overview Soluble in ethanol (20 mM) and DMSO (50 mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Chemical name (3S,6R,7E,9R,10R,12R,14S,15E,17E,19E,21S,23S,26R,27R,34aS)-9,10,12,13,14,21,22,23,24,25,26,27,32,33,34,34a-Hexadecahydro-9,27-dihydroxy-3-[(1R)-2-[(1S,3R,4R)-4-hydroxy-3-methoxycyclohexyl]-1-methylethyl]-10,21-dimethoxy-6,8,12,14,20,26-hexamethyl-23,27-epoxy-3H-pyrido[2,1-c][1,4]oxaazacyclohentriacontine-1,5,11,28,29(4H,6H,31H)-pentone
Molecular Weight 914.18
Chemical structure Rapamycin [ 53123-88-9 ] Chemical Structure
Molecular Formula C51H79NO13
CAS Number 53123-88-9
PubChem identifier 5284616
SMILES C[C@@H]1CC[C@H]2C[C@@H](/C(=C/C=C/C=C/[C@H](C[C@H](C(=O)[C@@H]([C@@H](/C(=C/[C@H](C(=O)C[C@H](OC(=O)[C@@H]3CCCCN3C(=O)C(=O)[C@@]1(O2)O)[C@H](C)C[C@@H]4CC[C@H]([C@@H](C4)OC)O)C)/C)O)OC)C)C)/C)OC
InChiKey QFJCIRLUMZQUOT-HPLJOQBZSA-N
MDL number MFCD00867594
Appearance White to off-white

References for Rapamycin

References are publications that support the biological activity of the product
  • Targeting T-cell senescence and cytokine storm with rapamycin to prevent severe progression in COVID-19

    Mahe et al (2020) Clin Immunol. 216 : 108464
  • A split-Cas9 architecture for inducible genome editing and transcription modulation.

    Zetsche et al (2015) Nat Biotechnol 33(2) : 139-42
  • Chemical modulators of autophagy as biological probes and potential therapeutics.

    Flemin et al (2011) Nat Chem Biol 7(1) : 9-17
  • Rapamycin, a specific inhibitor of the mammalian target of rapamycin, suppresses lymphangiogenesis and lymphatic metastasis.

    Kobayashi et al (2007) Cancer Sci 98(5) : 726-33
  • Rapamycins: mechanism of action and cellular resistance.

    Huang et al (2003) Cancer Biol Ther 2(3) : 222-32
Publications
These publications cite the use of Rapamycin purchased from Hello Bio:
  • The Effects of Rapamycin on the Intestinal Graft in a Rat Model of Cold Ischemia Perfusion and Preservation.

    Caleb I et al (2022) Metabolites 12
    PubMedID: 36144199
  • Characterizing Autophagy in the Cold Ischemic Injury of Small Bowel Grafts: Evidence from Rat Jejunum

    Caleb I et al (2021) Metabolites 11(6)
    PubMedID: 34204418
  • NMDA receptor partial agonist GLYX-13 alleviates chronic stress-induced depression-like behavior through enhancement of AMPA receptor function in the periaqueductal gray

    Yang PS et al (2020) Neuropharmacology 178 : 108269
    PubMedID: 32791085

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