Product overview
Name | Picrotoxin |
Description | Non-competitive GABAA receptor antagonist |
Alternative names | PTX |
Purity | >98% |
Customer comments | Getting on well with DHPG & picrotoxin – they do what they’re supposed to! Professor Bruno Frenguelli, University of Warwick, UK Reasonable price and good working! Verified customer, Seoul National University |
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Biological Data
Biological description | Non-competitive GABAA receptor antagonist. Also a glycine receptor inhibitor (IC50 = 2.7 µM). Acts as a convulsant and CNS stimulant. Active in vivo. |
Application notes | The GABAA receptor antagonist Picrotoxin is commonly used to reduce the levels inhibition by blocking the actions of the neurotransmitter GABA. Picrotoxin from Hello Bio reduces both spontaneous inhibitory post synaptic currents (IPSC) and evoked IPSCs. It was effective at concentrations of 10μM, with complete receptor blockade at 100μM. For assay protocol, see #Protocol 1 in Application Notes below
#Protocol 1: Evoked and spontaneous inhibitory post synaptic currents (IPSCs)
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Solubility & Handling
Storage instructions | Room temperature |
Solubility overview | Soluble in DMSO (100mM) and in ethanol (50mM, gentle warming) |
Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
Molecular Weight | 602.59 |
Chemical structure | |
Molecular Formula | C30H34O13 |
CAS Number | 124-87-8 |
PubChem identifier | 518601 |
SMILES | CC(=C)C1C2C3C4(C(C1C(=O)O2)(CC5C4(O5)C(=O)O3)O)C.CC12C3C4C(C(C1(CC5C2(O5)C(=O)O3)O)C(=O)O4)C(C)(C)O |
InChi | InChI=1S/C15H18O7.C15H16O6/c1-12(2,18)6-7-10(16)20-8(6)9-13(3)14(7,19)4-5-15(13,22-5)11(17)21-9;1-5(2)7-8-11(16)19-9(7)10-13(3)14(8,18)4-6-15(13,21-6)12(17)20-10/h5-9,18-19H,4H2,1-3H3;6-10,18H,1,4H2,2-3H3 |
InChiKey | VJKUPQSHOVKBCO-UHFFFAOYSA-N |
MDL number | MFCD00074824 |
References for Picrotoxin
References are publications that support the biological activity of the product
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Picrotoxin-like channel blockers of GABAA receptors.
Olsen RW (2006) Proc Natl Acad Sci U S A 103(16) : 6081-2. -
Mechanisms for picrotoxin block of alpha2 homomeric glycine receptors.
Wang DS et al (2006) J Biol Chem 281(7) : 3841-55. -
Picrotoxin blockade of invertebrate glutamate-gated chloride channels: subunit dependence and evidence for binding within the pore.
Etter A et al (1999) J Neurochem 72(1) : 318-26.
Publications
These publications cite the use of Picrotoxin purchased from Hello Bio:
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The impact of two autism related genes on amygdala physiology
Toft, A. (2019) The University of Edinburgh Thesis : 1-228 -
Excitatory neuron-specific SHP2-ERK signaling network regulates synaptic plasticity and memory.
Ryu et al (2019) Sci Signal. 12(571) : pii: eaau5755PubMedID: 30837304 -
KCC2 regulates neuronal excitability and hippocampal activity via interaction with Task-3 channels
Goutierre M et al (2019) Cell Rep 28(1) : 91-103.e7PubMedID: 31269453 -
Positive regulation of raphe serotonin neurons by serotonin 2B receptors.
Belmer et al (2018) Neuropsychopharmacology doi: 10.1038/s41386-018-0013-0. : [Epub ahead of print]PubMedID: 29453444 -
Second-hit mosaic mutation in mTORC1 repressor DEPDC5 causes focal cortical dysplasia-associated epilepsy.
Ribierre et al (2018) J Clin Invest. 128(6) : 2452-2458PubMedID: 29708508