Figure 1. DL-AP5 sodium salt inhibition of evoked NMDAR mediated EPSCs in rat CA1 pyramidal neuron
DL-AP5 sodium salt is a water soluble NMDA receptor antagonist. It is typically used at a concentration of 50–100 µM. DL-AP5 sodium salt from Hello Bio reduces evoked NMDAR current with full receptor antagonism at 50 µM, consistent with the literature for this compound. For assay protocol, see #Protocol 1 in Application Notes below.
DL-AP5 sodium salt: Scientist Approved
DL-AP5 sodium salt product vial image | Hello Bio
Figure 1. DL-AP5 sodium salt inhibition of evoked NMDAR mediated EPSCs in rat CA1 pyramidal neuron
DL-AP5 sodium salt is a water soluble NMDA receptor antagonist. It is typically used at a concentration of 50–100 µM. DL-AP5 sodium salt from Hello Bio reduces evoked NMDAR current with full receptor antagonism at 50 µM, consistent with the literature for this compound. For assay protocol, see #Protocol 1 in Application Notes below.
DL-AP5 sodium salt: Scientist Approved
DL-AP5 sodium salt product vial image | Hello Bio
Biological Data
Biological description
DL-AP5 sodium salt is a water soluble, competitive NMDA receptor antagonist and is the sodium salt of DL-AP5. DL-AP5 sodium salt binds at the glutamate site and impairs learning and fear conditioning.
Application notes
DL-AP5 sodium salt is a water soluble NMDA receptor antagonist. It is typically used at a concentration of 50–100 µM. DL-AP5 sodium salt from Hello Bio reduces evoked NMDAR current with full receptor antagonism at 50 µM (see Fig 1 above), consistent with the literature for this compound.
#Protocol 1: Evoked NMDAR currents at +40 mV
NMDAR currents were recorded via whole cell voltage clamp recordings of CA1 pyramidal neurons from rat hippocampal brain slice.
NMDAR currents were evoked via a stimulating electrode placed in the CA3 region to stimulate the Schaffer collateral pathway.
Each NMDAR current was evoked via a single square (150 µs) pulse every 10 sec at a stimulus intensity that gave a reliable NMDAR current.
Neurons were constantly held at +40 mV and NMDAR currents recorded in response to continual bath applications of NMDAR antagonists.
All NMDAR recordings were made in the presence of GABAA-R and AMPAR antagonists.
Solubility & Handling
Storage instructions
Room temperature (desiccate)
Solubility overview
Soluble in water (100mM)
Handling
Hydroscopic solid, contact with air may cause material to become sticky. Product performance should not be affected but we recommend storing the material in a sealed jar.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
References are publications that support the biological activity of the product
Infusion of the NMDA receptor antagonist, DL-APV, into the basolateral amygdala disrupts learning to fear a novel and a familiar context as well as relearning to fear an extinguished context.
These publications cite the use of DL-AP5 sodium salt purchased from Hello Bio:
The positive allosteric modulator of NMDA receptors, GNE-9278, blocks the ethanol-induced decrease of excitability in developing retrosplenial cortex neurons from mice.
Bird CW et al (2023) Neuropsychopharmacology reports 43 : 77-84
![DL-AP5 sodium salt [1303993-72-7] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb0252.png "DL-AP5 sodium salt [1303993-72-7]")
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