Deschloroclozapine dihydrochloride (DCZ) (water soluble)

Technical documents: SDS CoA Datasheet

Product overview

Name Deschloroclozapine dihydrochloride (DCZ) (water soluble)
Alternative names DCZ
Purity >99%
Description Potent, selective and metabolically stable hM3Dq and hM4Di muscarinic DREADD actuator. Water soluble.
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Biological Data

Biological description


Deschloroclozapine (DCZ) is reported to be a potent, selective and highly brain-penetrable muscarinic hM3Dq and hM4Di DREADD actuator with minimal off-target actions (Ki = 6.3 and 4.2 nM at hM3Dq and hM4Di respectively) and (EC50 values are 0.13 and 0.081 nM at hM3Dq and hM4Di respectively in a BRET-based assay.

It represents a potent, selective, metabolically stable and fast acting DREADD agonist with utility in both mice and non-human primates for a variety of applications.

It shows 100-fold improved affinity and greater agonist potency for hM3Dq and hM4Di compared to Clozapine n-Oxide (CNO) or DREADD agonist 21 (C21) with reduced off-target binding compared with clozapine in vitro.It has lower affinity at D1, D2 and 5-HT2A and 5-HT2C receptors compared with clozapine.

PET studies demonstrate the compound is rapidly brain penetrable, is apparently selective and doses for DREADD occupancy are 20-fold and 60-fold lower than CNO or DREADD agonist 21 (C21) respectively.

Uses and applications

Systemic delivery of low doses of DCZ (1 or 3 µg/kg) were shown to enhance neuronal activity via hM3Dq in mice and monkeys within minutes.

Intramuscular doses of 100 µg/kg reversibly induced spatial working memory deficits in hM4Di expressing monkeys.

Deschloroclozapine freebase also available.

Solubility & Handling

Storage instructions -20°C
Solubility overview Soluble in water (100 mM). Always store solutions at -20°C.

Storage of solid

  • Store at -20°C.
  • Please note that the compound is a hydroscopic solid and contact with air may cause material to become sticky. Product performance should not be affected but we recommend storing the material in a sealed jar.

Storage of solutions

  • Make up solutions and use immediately.
  • If storage of solutions is required, you should aliquot out the solution into tightly sealed vials and store at -20°C and store these for up to one month.
  • Allow the product to equilibrate to RT for at least one hour before opening and using.

Storage of solutions at room temperature

  • We recommend only keeping solutions at room temperature (25°C) for a few days as our studies have shown that after 96 hours the purity of the compound in solution drops to ~97% and will continue to drop over time.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use



More Info


More Info

Chemical Data

Purity >99%
Chemical name 6-(4-methylpiperazin-1-yl)-11H-benzo[b][1,4]benzodiazepine dihydrochloride
Molecular Weight 365.3
Chemical structure Deschloroclozapine dihydrochloride [1977-07-7] Chemical Structure
Molecular Formula C18H20N4.2HCl
CAS Number 1977-07-7 (free base)
SMILES Cl.Cl.CN1CCN(CC1)C2=Nc4ccccc4Nc3ccccc23
Source Synthetic
InChi InChI=1S/C18H20N4.2ClH/c1-21-10-12-22(13-11-21)18-14-6-2-3-7-15(14)19-16-8-4-5-9-17(16)20-18;;/h2-9,19H,10-13H2,1H3;2*1H
Appearance Yellow solid

References for Deschloroclozapine dihydrochloride (DCZ) (water soluble)

References are publications that support the biological activity of the product
  • Deschloroclozapine, a potent and selective chemogenetic actuator enables rapid neuronal and behavioral modulations in mice and monkeys

    Nagai et al (2020) Nature Neuroscience : 1157-1167
  • Chemogenetic actuator drugs impair prefrontal cortex-dependent working memory in rhesus monkeys

    Upright and Baxter (2019) bioRxiv :
  • Binding of 5H-dibenzo[b,e][1,4]diazepine and chiral 5H-dibenzo[a,d]cycloheptene analogues of clozapine to dopamine and serotonin receptors.

    Phillips et al (1994) J Med Chem 37(17) : 2686-96

3 Item(s)

These publications cite the use of Deschloroclozapine dihydrochloride (DCZ) (water soluble) purchased from Hello Bio:
  • The use of chemogenetic actuator ligands in nonhuman primate DREADDs-fMRI

    Cushnie et al (2022) Current Research in Neurobiology
    PubMedID: 36691404
  • Oestrogen engages brain MC4R signalling to drive physical activity in female mice

    Krause et al (2021) Nature. 599 : 131-135
    PubMedID: 34646010
  • Tissue-specific modification of cellular bioelectrical activities using the chemogenetic tool, DREADD, in zebrafish

    Zhang et al (2021) bioRxiv : doi
  • Estrogen Drives Melanocortin Neurons To Increase Spontaneous Activity and Reduce Sedentary Behavior

    Krause et al (2021) bioRxiv doi: :
  • Behavioral and slice electrophysiological assessment of DREADD ligand, deschloroclozapine (DCZ) in rats

    Nentwig et al (2021) bioRxiv : .

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