CNQX

(HB0204)
Technical documents: SDS CoA Datasheet

Product overview

Name CNQX
Description Potent, competitive AMPA / kainate receptor antagonist
Purity >98%
Customer comments

Absolute satisfaction not only with item received, but especially with communication. This product (CNQX) meets our expectation and description as declared on websites. Communication was immediate and fast. Item came second day after purchase has been done, firmly packed. Unrivaled prices, friendly approach! Verified customer, National institute of mental health Czech Republic

Another quality compound! CNQX from Hello Bio works great in our experiments and at a price that is definitely nice! We continue to be impressed with the value we get from this company. Verified customer, University of South Carolina

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Images

CNQX inhibition of AMPA receptor mediated EPSCs
CNQX inhibition of glutamate stimulated calcium fluorescence
CNQX: Scientist Approved
CNQX - vial

Biological Data

Biological description

Potent and competitive AMPA and kainate receptor antagonist. Also antagonises NMDA receptors at the glycine site. Increases GABAA receptor spontaneous postsynaptic currents (sPSCs). Shows neuroprotective actions. Water soluble, CNQX disodium salt also available.

Application notes

The AMPA receptor antagonist CNQX is commonly used at concentrations of 10 μM to inhibit the actions of glutamate acting on AMPARs.

CNQX from Hello Bio reduces both spontaneous and evoked EPSCs in cortical neurons at concentrations of 1 μM with full AMPA receptor blockade at 10 μM (see Fig 1 above).

 

#Protocol 1: Evoked and spontaneous excitatory post synaptic currents (EPSCs)

  • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
  • EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
  • Neurons were held at -70 to -60 mV (the reversal potential of GABA currents). EPSCs were continuously stimulated and recorded in response to 5 min applications of varying concentrations of CNQX until complete receptor inhibition.
  • Spontaneous EPSCs were recorded before and after addition of CNQX by holding the neuron at -70 mV and recording for 10 sec.
  • Recordings for EPSCs were made in the absence of GABAA-R antagonists.

Solubility & Handling

Storage instructions Room temperature
Solubility overview Soluble in DMSO (100mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Chemical name 6-Cyano-7-nitroquinoxaline-2,3-dione
Molecular Weight 232.16
Chemical structure CNQX  [115066-14-3] Chemical Structure
Molecular Formula C9H4N4O4
CAS Number 115066-14-3
PubChem identifier 3721046
SMILES C1=C(C(=CC2=C1NC(=O)C(=O)N2)[N+](=O)[O-])C#N
Source Synthetic
InChi InChI=1S/C9H4N4O4/c10-3-4-1-5-6(2-7(4)13(16)17)12-9(15)8(14)11-5/h1-2H,(H,11,14)(H,12,15)
InChiKey RPXVIAFEQBNEAX-UHFFFAOYSA-N
MDL number MFCD00069232
Appearance Yellow solid

References for CNQX

References are publications that support the biological activity of the product
  • Dop neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons

    Chuhma et al (2018) eLIFE 7:e39786 : DOI: 10.7554/eLife.39786
  • The calpain inhibitor MDL-28170 and the AMPA/KA receptor antagonist CNQX inhibit neurofilament degradation and enhance neuronal survival in kainic acid-treated hippocampal slice cultures.

    Lopez-Picon FR et al (2006) Eur J Neurosci 23(10) : 2686-94.
  • 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX) increases GABAA receptor-mediated spontaneous postsynaptic currents in the dentate granule cells of rat hippocampal slices.

    Hashimoto Y et al (2004) Neurosci Lett 358(1) : 33-6.
  • Pharmacological characterization of glutamatergic agonists and antagonists at recombinant human homomeric and heteromeric kainate receptors in vitro.

    Alt et al (2004) Neuropharmacology 46(6) : 793-806
  • 6,7-Dinitro-quinoxaline-2,3-dion and 6-nitro,7-cyano-quinoxaline-2,3-dion antagonise responses to NMDA in the rat spinal cord via an action at the strychnine-insensitive glycine receptor.

    Birch PJ et al (1988) Eur J Pharmacol 156(1) : 177-80.
Publications
These publications cite the use of CNQX purchased from Hello Bio:
  • Activation of TRPC1 Channel by Metabotropic Glutamate Receptor mGluR5 Modulates Synaptic Plasticity and Spatial Working Memory

    Lepennetier et al (2018) Front. Cell. Neurosci. https://doi.org/ : 10.3389/fncel.2018.00318
  • Artifactual hyperpolarization during extracellular electrical stimulation: Proposed mechanism of high-rate neuromodulation

    Lesperance et al (2017) Brain stimulation xxx : 1-10
  • SALM5 trans-synaptically interacts with LAR-RPTPs in a splicing-dependent manner to regulate synapse development.

    Choi et al (2016) Sci Rep 26 : 6:26676
    PubMedID: 27225731

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