Your Top Technical Questions Answered - How to Solubilise CNO Dihydrochloride
At Hello Bio, we’re proud to offer high-quality life science reagents, fair prices, and fast global delivery — backed by expert scientific support. When researchers ask us technical questions about any of our products, our PhD-qualified team is always ready to help.
In this feature we answer a common question about one of our most popular DREADD ligands — CNO dihydrochloride (HB6149). Cited in numerous peer-reviewed papers, this water-soluble Clozapine N-oxide is a go-to chemogenetic tool trusted by labs worldwide.
★ Your Top Technical Question
“We have recently purchased CNO from Hello Bio. Please can you advise the best method to solubilise the product in water effectively?”
Happy to help! Clozapine-N-oxide (CNO) dihyrochloride is a powerful tool for chemogenetic research, but only when solubilised correctly.
Here is our recommended method for effective solubilisation using water as a solvent:
1. Use our free molarity calculator to work out the exact volume of water and mass of CNO you’ll need to achieve your desired concentration
2. Remember that CNO is hygroscopic which means it can absorb moisture from the air. If your vial has been stored frozen, ensure it returns to room temperature before opening to avoid any solubility issues.
3. Carefully pipette your calculated volume of water into a vial containing your CNO. Make sure to pipette slowly down the side of the vial to avoid splashing or loss of material.
4. If any solid remains undissolved, gently vortex the vial to help the CNO dissolve fully.
5. When there is no visible solid remaining, your CNO has been solubilised and is ready to use!
What is CNO Dihydrochloride Used For?
CNO dihydrochloride is widely used in neuroscience and behavioral research as a selective chemogenetic actuator - it selectively modulates neuronal activity in DREADD (Designer Receptors Exclusively Activated by Designer Drugs) systems.
CNO enables researchers to reversibly activate or inhibit targeted neurons by binding to these designer receptors without affecting endogenous receptor systems. This makes it particularly valuable in studies of neural circuits, behavior, and neuropsychiatric disorders. The dihydrochloride form is water-soluble, more stable and easier to handle than the freebase form, making it highly suitable for in vivo studies.
View CNO dihydrochloride (HB6149)
More About CNO & DREADDS
- View CNO Dihydrochloride (HB6149)
- Browse our full range of Chemogenetic Compounds & DREADD ligands
- Stability of Water-Soluble DREADD Ligands: A Technical Review
More Help from Our Team
Have a technical question about CNO, DREADDs, or any other Hello Bio product? Our technical team is here to help.
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