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Technical documents: SDS CoA Datasheet

Product overview

Name Biocytin
Biological description


Biocytin is a conjugate of biotin and lysine which is small and soluble. It is widely used in neuroanatomical research as a neuroanatomical tracer.

Uses and applications

Biocytin is taken up by neurons and rapidly transported in both anterograde and retrograde directions.

It has high affinity for avidin and consequently, can be visualized by various avidin and streptavidin-conjugated markers for light, fluorescence or electron microscope detection.

Biocytin has a short half-life and is degraded by biotinidase a few hours after application.

Purity >95% (NMR)
Description Classical neuroanatomical tracer
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Biocytin product vial image | Hello Bio

Solubility & Handling

Storage instructions -20°C
Solubility overview Soluble in water (50 mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use



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Chemical Data

Purity >95% (NMR)
Chemical name N6-[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]-L-lysine
Molecular Weight 372.5
Chemical structure Biocytin | [576-19-2] Chemical Structure
Molecular Formula C16H28N4O4S
CAS Number 576-19-2
PubChem identifier 83814
SMILES C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCC[C@@H](C(=O)O)N)NC(=O)N2
InChi InChI=1S/C16H28N4O4S/c17-10(15(22)23)5-3-4-8-18-13(21)7-2-1-6-12-14-11(9-25-12)19-16(24)20-14/h10-12,14H,1-9,17H2,(H,18,21)(H,22,23)(H2,19,20,24)/t10-,11-,12-,14-/m0/s1
MDL number MFCD00077319

References for Biocytin

References are publications that support the biological activity of the product
  • Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers

    Swietek B et al (2016) J Vis Exp 31 : 118
  • Biocytin-labelling and its impact on late 20th century studies of cortical circuitry

    Thomson AM et al (2011) Brain Res Rev 66(1-2) : 43-53
  • Neuroanatomical labeling with biocytin: a review

    McDonald AJ (1992) Neuroreport 3(10) : 821-7

3 Item(s)

These publications cite the use of Biocytin purchased from Hello Bio:
  • Ventral tegmental area astrocytes modulate cocaine reward by tonically releasing GABA.

    Yang J et al (2023) Neuron 111 : 1104-1117.e6
    PubMedID: 36681074
  • Local circuit allowing hypothalamic control of hippocampal area CA2 activity and consequences for CA1

    Robert V et al (2021) Elife 10
    PubMedID: 34003113

2 Item(s)