Biotinyl Tyramide

Biotinyl tyramide is widely used as part of the Tyramide signal amplification protocol. Target proteins are labelled with a horseradish peroxidase (HRP) labelled antibody and in the presence of H₂O₂ the biotinyl tyramide is covalently attached to tyrosine residues on nearby proteins. This provides localised signal amplification while still maintaining good spatial resolution and the biotin can then be detected with fluorescently labelled streptavidin.

-20°C

Soluble in DMSO (100mM, 36.35mg/ml)

5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[2-(4-hydroxyphenyl)ethyl]pentanamide

C₁₈H₂₅N₃O₃S