Biotinyl tyramide is widely used as part of the Tyramide signal amplification protocol in immunohistochemistry (IHC), in-situ hybridisation (ISH) and APEX proximity labeling. Target proteins are labelled with a horseradish peroxidase (HRP) labelled antibody and in the presence of H2O2 the biotinyl tyramide is covalently attached to tyrosine residues on nearby proteins. This provides localised signal amplification while still maintaining good spatial resolution and the biotin can then be detected with fluorescently labelled streptavidin. Suitable for use with ascorbate peroxidase (APEX) proximity labelling to biotinylate interaction partners of the APEX-tagged protein for downstream proteomic analysis.
Solubility & Handling
Solubility overview
Soluble in DMSO (100mM, 36.35mg/ml)
Storage instructions
-20°C
Storage of solutions
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
