Good quality product: (-)-Bicuculline methiodide is used routinely in our lab for a number of experiments. It is shipped quickly, packaged well, dissolves without problem, and blocks GABAA-receptor activity as it should! Verified customer, Sickkids foundation
We routinely use this compound from Hello Bio to inhibit GABA-A receptors in electrophysiological recordings from rodent brain slices. Verified customer, University of Montana
Figure 1. Bicuculline methiodide inhibition of evoked and spontaneous GABAA-R mediated IPSCs in mouse cortical neurons
The GABAA receptor antagonist bicuculline is commonly used to reduce levels of inhibition by blocking the actions of the neurotransmitter GABA. Bicuculline is commonly used at concentrations of 100 µM and above Bicuculline methiodide from Hello Bio reduces both spontaneous inhibitory post synaptic currents (IPSC) and evoked IPSCs. It was effective at concentrations of 1 µM with complete receptor blockade at 100 µM For assay protocol, see #Protocol 1 in Application Notes below
Figure 2. Bicuculline methiodide inhibition of spontaneous IPSCs in cultured
rat cortical neurons
Spontaneous IPSCs were recorded before and after addition of Bicuculline methiodide. Primary cultured rat cortical neurons, DIV 21-22, were voltage clamped at 0mV and spontaneous inhibitory postsynaptic currents were recorded. Recordings were made in the presence of D-AP5 and NBQX.
(-)-Bicuculline methiodide product vial image | Hello Bio
Figure 1. Bicuculline methiodide inhibition of evoked and spontaneous GABAA-R mediated IPSCs in mouse cortical neurons
The GABAA receptor antagonist bicuculline is commonly used to reduce levels of inhibition by blocking the actions of the neurotransmitter GABA. Bicuculline is commonly used at concentrations of 100 µM and above Bicuculline methiodide from Hello Bio reduces both spontaneous inhibitory post synaptic currents (IPSC) and evoked IPSCs. It was effective at concentrations of 1 µM with complete receptor blockade at 100 µM For assay protocol, see #Protocol 1 in Application Notes below
Figure 2. Bicuculline methiodide inhibition of spontaneous IPSCs in cultured
rat cortical neurons
Spontaneous IPSCs were recorded before and after addition of Bicuculline methiodide. Primary cultured rat cortical neurons, DIV 21-22, were voltage clamped at 0mV and spontaneous inhibitory postsynaptic currents were recorded. Recordings were made in the presence of D-AP5 and NBQX.
(-)-Bicuculline methiodide product vial image | Hello Bio
The GABAA receptor antagonist bicuculline is commonly used to reduce levels of inhibition by blocking the actions of the neurotransmitter GABA. Bicuculline is commonly used at concentrations of 100 μM and above.
Bicuculline methiodide from Hello Bio reduces both spontaneous inhibitory post synaptic currents (IPSC) and evoked IPSCs (see Fig 1 above). It was effective at concentrations of 1 mM with complete receptor blockade at 100 μM.
#Protocol 1: Evoked and spontaneous inhibitory post synaptic currents (IPSCs)
Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
A stimulating electrode was placed in layers II/III and IPSCs were evoked by a single square (150 μs) pulse every 10 sec at a stimulus intensity that gave a reliable IPSC.
IPSCs were evoked at a range of neuron holding voltages to measure the reversal potential of the current to ensure it was GABAergic.
Neurons were held at 0mV and IPSCs continuously stimulated and recorded in response to 5 min applications of varying concentrations of Bicuculline methiodide until complete receptor inhibition.
Spontaneous IPSCs were recorded before and after addition of Bicuculline methiodide by holding the neuron at 0mV and recording for 10 sec.
All recordings for IPSCs were made in the presence of AMPAR antagonists.
Solubility & Handling
Storage instructions
Room temperature
Solubility overview
Soluble in water (20mM) or DMSO (50mM)
Handling
This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
These publications cite the use of (-)-Bicuculline methiodide purchased from Hello Bio:
Activation of TRPC1 Channel by Metabotropic Glutamate Receptor mGluR5 Modulates Synaptic Plasticity and Spatial Working Memory
Lepennetier et al (2018) Front. Cell. Neurosci. https://doi.org/ : 10.3389/fncel.2018.00318
Activation of Serotonin 5-HT7 Receptors Modulates Hippocampal Synaptic Plasticity by Stimulation of Adenylate Cyclases and Rescues Learning and Behavior in a Mouse Model of Fragile X Syndrome.
Costa et al (2018) Front Mol Neurosci. 11:353 : doi: 10.3389/fnmol.2018.00353
![(-)-Bicuculline methiodide [40709-69-1] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb0893.png "(-)-Bicuculline methiodide [40709-69-1]")
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