Figure 1. Astrocytes in culture in a cultured rat neuron preparation.
Figure 2. Astrocyte populations in culture stained by HB7592.
Figure 3. Astrocytes stained for GFAP with HB7592 in the cerebellum
Figure 4. Independent antibody validation of HB7592 and HB6406.
Figure 5. Concentration response of HB7592 staining in cultured rat neurons.
Figure 1. Astrocytes in culture in a cultured rat neuron preparation.
Figure 2. Astrocyte populations in culture stained by HB7592.
Figure 3. Astrocytes stained for GFAP with HB7592 in the cerebellum
Figure 4. Independent antibody validation of HB7592 and HB6406.
Figure 5. Concentration response of HB7592 staining in cultured rat neurons.
Product information
Immunogen
Recombinant human GFAP (isoform 1) expressed in and purified from E. coli
Purification
Immunogen affinity purification
Concentration
1mg/ml
Formulation
50% PBS, 50% glycerol + 5mM sodium azide
Predicted species reactivity
Mouse, Rat, Human
Tested species reactivity
Mouse, Rat
Tested applications
Applications
ICC, IHC(IF)
IHC(IF) optimal concentration
0.5µg/ml (1:2,000 dilution) as tested in free-floating paraformaldehyde fixed rat brain sections
ICC optimal concentration
0.5µg/ml (1:2,000 dilution) as tested in cultured rat neurones
Positive control
GFAP is highly expressed in neural tissues containing astrocytes. It is not widely expressed in cell lines, however it is in specific lines such as U-87 MG.
Negative control
Most non-neural tissues. Please note that GFAP expression has been reported in a subset of pancreatic and hepatic cells in rats and mice kidney cells. It is generally poorly expressed in common cell lines such as HeLa or HEK293.
GFAP has three confirmed and 21 potential isoforms. Isoform 1 (GFAP alpha): canonical, 49.9kDa; Isoform 2 (GFAP epsilon): amino acid changes between positions 391 and 432, 49.5kDa; Isoform 3 (GFAP kappa): amino acid changes between positions 391 and 432, 50.3kDa
Expression
GFAP is primarily expressed within astrocytes of the central nervous system alongside also expressing in non-myelinating Schwann cells of the peripheral nervous system and satellite cells of the peripheral ganglia. GFAP expression has also been reported in Leydig cells of the testis alongside stellate cells from the pancreas and liver in rats.
Subcellular expression
GFAP is a key cytoskeletal component therefore is widely expressed as bundles of GFAP positive fibres.
Processing
Following translation, no processing is required for GFAP to reach its active conformation.
Post translational modifications
GFAP is subjected to numerous post-translational modifications including 9 phosphorylation sites which are the target of AURKB and ROCK1 alongside 5 separate citrullination sites.
Homology (compared to human)
Rat, mouse and human GFAP proteins have a 90% similarity score in a direct BLAST comparison.
Similar proteins
Other type III intermediate filament proteins have homology with GFAP including Vimentin (58%), Desmin (59%) and Peripherin (56%) when assessed using BLAST.
Storage & Handling
Storage instructions
-20°C
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
GFAP (Glial Fibrillary Acidic Protein) is a marker for astrocytes, which are a type of glial cell in the central nervous system (CNS). GFAP is an intermediate filament protein expressed predominantly in mature astrocytes and is commonly used as a marker in research and diagnostic pathology to identify these cells.
What is the function of GFAP?
Thefunction of GFAP (Glial Fibrillary Acidic Protein) is primarily structural and supportive. It is an intermediate filament protein that contributes to the cytoskeletal structure of astrocytes in the central nervous system (CNS).
What is the role of GFAP in neurological diseases?
GFAP (Glial Fibrillary Acidic Protein) plays a significant role in various neurological diseases due to its involvement in the structural and functional integrity of astrocytes. Changes in GFAP expression are associated with:
Reactive gliosis in CNS injury, often as a result of acute trauma leading to a glial scar,
Lesions found in multiple sclerosis which have high GFAP expression,
Astrocytomas and Glioblastimas can often express GFAP which can be used as a diagnostic marker
Neurodegeneration is often associated with upregulation of GFAP expression in damaged brain regions.
What mounting media do you recommend to use with this antibody?
We recommend using one of our high performance mounting medias, supplied as either hardset or aqeous with a range of counterstains:
What guarantee do you have that my GFAP antibody will perform as expected?
We guarantee that your GFAP antibody will work for the applications and species we list on the datasheet. If the antibody fails to perform as expected then we are happy to offer a 100% refund guarantee. For more details please see our guarantee policy.
Will my GFAP antibody work against species that have not been listed on the datasheet?
A species not being listed doesn’t mean that the GFAP antibody won’t work, just that we haven’t tested it. If you test one of our antibodies in a new species please let us know (positive or negative)!
What protocols are available for use with this GFAP antibody
We have made a comprehensive collection of protocols that we have used in our experiments to validate this GFAP antibody.
Western Blot Protocol (1 MB) 
