As this product is a toxin, customers are required to complete a short end user declaration when ordering. Our customer care team will be happy to help you with this.
This is a home office notifiable schedule 5 toxin.
Customer comments
Just washed the Tetrodotoxin (TTX) on and it works as expected (it inhibits the fibre volley at 1 μM).Verified customer, The University of Bristol
Tetrodotoxin (citrate free) is a wonderful product. Very efficient and very effective in blocking sodium channels.Verified customer, Drexel University College of Medicine
Figure 1. TTX mediated inhibition of action potential firing upon post synaptic current injection in cortical neuron
Tetrodotoxin is commonly used in electrophysiology to block excitability by inhibiting action potential firing within neurons. It is commonly applied at concentrations of 1 µM. Tetrodotoxin from Hello Bio works as expected and blocks action potential firing. For assay protocol, see #Protocol 1 in Application Notes below.
Figure 2. TTX mediated inhibition of electrically evoked EPSP and action potential within cortical neuron
Tetrodotoxin is commonly used in electrophysiology to block excitability by inhibiting action potential firing within neurons. It is commonly applied at concentrations of 1 µM. Tetrodotoxin from Hello Bio works as expected and blocks action potential firing For assay protocol, see #Protocol 2 in Application Notes below.
Tetrodotoxin (citrate free): Scientist Approved
Figure 1. TTX mediated inhibition of action potential firing upon post synaptic current injection in cortical neuron
Tetrodotoxin is commonly used in electrophysiology to block excitability by inhibiting action potential firing within neurons. It is commonly applied at concentrations of 1 µM. Tetrodotoxin from Hello Bio works as expected and blocks action potential firing. For assay protocol, see #Protocol 1 in Application Notes below.
Figure 2. TTX mediated inhibition of electrically evoked EPSP and action potential within cortical neuron
Tetrodotoxin is commonly used in electrophysiology to block excitability by inhibiting action potential firing within neurons. It is commonly applied at concentrations of 1 µM. Tetrodotoxin from Hello Bio works as expected and blocks action potential firing For assay protocol, see #Protocol 2 in Application Notes below.
Tetrodotoxin (citrate free): Scientist Approved
Biological Data
Biological description
Potent, selective and reversible, voltage dependent Na+ channel blocker. Use dependent. Commonly used in electrophysiological preparations to block or reduce excitability. Water soluble, Tetrodotoxin citrate also available.
Application notes
Tetrodotoxin is commonly used in electrophysiology to block excitability by inhibiting action potential firing within neurons. It is commonly applied at concentrations of 1 μM. Tetrodotoxin from Hello Bio works as expected and blocks action potential firing (see Fig 1 and 2 above).
#Protocol 1: Effect of TTX citrate on action potentials in mouse cortical neurons
Whole cell voltage clamp recordings were obtained from layer V pyramidal neurons of the mouse prelimbic cortex brain slice.
Neurons were held at the resting membrane potential (~ -70 mV) and injected with a 500 pA 300 ms current step to induce action potential firing.
TTX was bath applied for 10 min first at 100 nM then 300 nM, 1 μM and 2 μM. After each drug application a current step was recorded to assess action potential blockade.
#Protocol 2: Effect of TTX citrate on EPSPs and action potential firing in mouse cortical neurons
Whole cell voltage clamp recordings were obtained from layer V pyramidal neurons of the mouse prelimbic cortex brain slice.
Neurons were held at the resting membrane potential (~ -70 mV) and EPSP were evoked by placing a stimulating electrode close to the recorded the neuron in layer II/III.
EPSPs and action potentials were evoked by single square (150 μs) pulse every 10 sec with an intensity that produced both an EPSP and action potential.
TTX was bath applied for 10 min first at 100 nM then 300 nM, 1 μM and 2 μM whist continually evoking and recording EPSP/A.Ps.
Solubility & Handling
Storage instructions
-20°C (desiccate)
Solubility overview
Soluble in acidic buffer (pH 4.8) to 3mM
Handling
This product is soluble to 3mM in an acidic buffer (pH 4.8), e.g. a 0.1M citrate or acetate buffer. Therefore, you can make a buffer solution by dissolving either sodium citrate or sodium acetate in water to a final concentration of 0.1M. You can then adjust the pH via the addition of citric acid, acetic acid or sodium hydroxide to pH 4.8.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
![Tetrodotoxin (TTX) [4368-28-9] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb1034_1.png "Tetrodotoxin (TTX) [4368-28-9]")
Understanding purity and quality - a guide for life scientists
