Product overview

Name TBS (25x) (pH 7.4)
Biological description

Tris Buffered Saline (TBS) wash buffer to remove non-specific binding. For use in immunoassays such as western blot, IHC, ICC, ELISA. Tween 20 may be added to promote more effective washings. The buffer is supplied as a 25X stock solution that should be diluted to 1X with distilled water. When diluted to 1x contains 130mM NaCl, 20mM Tris, 0.002% ProClin-300, pH7.4.

Description Tris Buffered Saline (TBS) wash buffer to remove non-specific binding.
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Biological Data

Application notes

This is a 25x buffer: to use, dilute in dH2O (40ml of 20x buffer + 960ml dH2O)

Solubility & Handling

Storage instructions Room temperature
Storage of solutions Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

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Dilution

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Chemical Data

Appearance Colorless solution