Tris base

(HB5504)

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Product overview

Name Tris base
Biological description

Widely used component of buffer solutions in molecular biology such as TBS (Tris-buffered saline), TAE (Tris acetate / EDTA) and TBE (Tris borate / EDTA) buffers. Due to having a pKa of ≈8.1 at 25°C it effectively buffers solutions from pH 7 to 9, a range suitable for the majority of biological processes. Highly water soluble.

Alternative names Tris, tromethamine, THAM, Trometamol, Tris(hydroxymethyl)aminomethane
Purity >99%
Description

Widely used buffer component in molecular biology

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Biological Data

Application notes

Common buffer recipes:

  • TBS: 20mM Tris, 150mM NaCl, pH7.4
  • TAE: 40mM Tris, 20mM acetic acid, 1mM EDTA, pH8.5
  • TBE: 89mM Tris, 89mM boric acid, 2mM EDTA, pH8.3

Solubility & Handling

Storage instructions

RT

Solubility overview

Soluble in dH2O to ≈50g/100ml.

Storage of solutions Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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Chemical Data

Purity >99%
Chemical name

2-amino-2-(hydroxymethyl)propane-1,3-diol

Molecular Weight 121.14
Chemical structure Tris base chemical structure Chemical Structure
Molecular Formula

C4H11NO3

CAS Number 77-86-1
PubChem identifier 6503
SMILES C(C(CO)(CO)N)O
InChi InChI=1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChiKey LENZDBCJOHFCAS-UHFFFAOYSA-N
MDL number MFCD00004679
Appearance White crystalline solid

References for Tris base

References are publications that support the biological activity of the product
  • Tris-base buffer: a promising new inhibitor for cancer progression and metastasis

    Ibrahim-Hashim A, Abrahams D, Enriquez-Navas PM, Luddy K, Gatenby RA, Gillies RJ. (2017) Cancer Med :
  • Tris-HCl: a standard buffer for use in the physiologic pH range.

    Durst RA, Staples BR (1972) Clin Chem :

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