Widely used component of buffer solutions in molecular biology such as TBS (Tris-buffered saline), TAE (Tris acetate / EDTA) and TBE (Tris borate / EDTA) buffers. Due to having a pKa of ≈8.1 at 25°C it effectively buffers solutions from pH 7 to 9, a range suitable for the majority of biological processes. Highly water soluble.
