SP600125 is a selective, cell-permeable, reversible and ATP-competitive JNK (c-Jun N-terminal Kinase) inhibitor (IC50 values are 40-90 µM at JNK1,2,3). SP600125 shows selectivity over a range of other kinases.
SP600125 dose-dependently inhibits phosphorylation of JNK, inhibits expression of the inflammatory genes (COX-2, IL-2, IFN-γ and TNF-α) and prevents apoptosis in many cell types and inhibits autophagy in HeLa cells.
In addition, SP600125 also shows neuronal protective effects and is active in vivo.
SP600125 is also commonly used as a stem cell modulator which prevents BMP9-induced osteogenic mesenchymal stem cells (MSCs) and primary bone marrow stromal cells differentiation and is frequently used in media to maintain stem cells in naive pluripotent state.
Solubility & Handling
Solubility overview
Soluble in DMSO (100mM) or ethanol (5mM, gentle warming)
Storage instructions
-20°C (desiccate)
Storage of solutions
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
Y-27632 is a selective ROCK inhibitor. Frequently used 3D growth matrix component and for production of organoids. Also used when inducing neurons from fibroblasts. Manufactured to a high degree of optical purity.
![SP 600125 [129-56-6] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb2234.png "SP 600125 [129-56-6]")
Understanding purity and quality - a guide for life scientists
