Recombinant mouse MANF protein

(HB6565)
Technical documents: CoA Datasheet

Product overview

Name Recombinant mouse MANF protein
Biological description

The mouse MANF neurotrophic factor belongs to the ARMET family and has been shown to have neuroprotective effects for dopaminergic neurons.

MANF expression is also induced during ER stress and is involved in protein quality control during ER stress.

Alternative names Recombinant Mouse Mesencephalic Astrocyte-Derived Neurotrophic Factor, Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich protein, Protein ARMET, Manf, Armet.
Purity >98%
Description Neurotrophic factor with neuroprotective effects
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Biological Data

Application notes ED50 = 10µg/ml (determined by a cell proliferation assay using rat C6 cells), corresponding to a specific activity of >1.00IU/mg.

Solubility & Handling

Storage instructions -20°C
Solubility overview To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 100μg/ml, which can then be diluted to make a working solution
Handling
  • Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
  • Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
  • For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Purity >98%
Molecular Weight 18.2
Source E. Coli.
Appearance White lyophilized powder (sterile filtered & freeze-dried)
Formulation Lyophilized from a 0.2μm filtered solution in PBS (pH 7.4)

References for Recombinant mouse MANF protein

References are publications that support the biological activity of the product
  • Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) Elevates Stimulus-Evoked Release of Dopamine in Freely-Moving Rats

    Renko JM et al (2018) Mol Neurobiol 55(8) : 6755-6768
  • Mesencephalic astrocyte-derived neurotrophic factor is neurorestorative in rat model of Parkinson's disease

    Voutilainen MH et al (2009) J Neurosci 29(30) : 9651-9
  • MANF: a new mesencephalic, astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons

    Petrova P et al (2003) J Mol Neurosci 20(2) : 173-88

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