QX 314 chloride

(HB1030)
Technical documents: SDS CoA Datasheet

Product overview

Name QX 314 chloride
Description Membrane impermeable Na+ channel blocker
Alternative names N-Ethyllidocaine chloride
Purity >98%
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Images

QX 314 chloride: Scientist Approved
QX 314 chloride product vial image | Hello Bio

Biological Data

Biological description

Membrane impermeable Na+ channel blocker. A quaternary derivative of lidocaine. Displays anesthetic properties. Active in vivo. QX 314 bromide also available.

Application notes

#Figure 1: QX 314 inhibition of action potentials in rat CA1 pyramidal neurones during dendritic plateau potentials

QX 314 is a membrane impermeable Na+ channel blocker commonly used to inhibit action potential formation. QX 314 from Hello Bio inhibits the ability for action potentials to generate in two different cells during dendritic plateau potentials at 1mM (see Fig 1 above).

 

#Protocol 1: Evoked plateau potentials in rat CA1 pyramidal neurones

 

  • Pyramidal neurones from adult Wistar rats were patched in CA1 using a KMeSO4 internal solution with and without the addition of 1mM QX 314 (HB1030).
  • Cells were first held in Vclamp at -70mV for 10 minutes to wash out LTP before being transferred to Iclamp (again at -70mV) where they were stimulated at a high stimulation intensity to generate plateau potentials.
  • Stimulation consisted of one single stimulation followed 400ms later by 5 stimulations at 100Hz.
  • Experiments took place in the presence of the GABAB antagonist GCP-55845 (1μM, HB0960) and 50µM PTX.
  • Throughout the experiment input current was adjusted to maintain the cell at -70mV ± 0.5mV.
  • Data is shown from two separate cells.

Solubility & Handling

Storage instructions Room temperature
Solubility overview Soluble in water (100mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

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Dilution

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Chemical Data

Chemical name N-(2,6-Dimethylphenylcarbamoylmethyl)triethylammonium chloride
Molecular Weight 298.85
Chemical structure QX 314 chloride  [1265634] Chemical Structure
Molecular Formula C16H27N2OCl
CAS Number 5369-03-9
PubChem identifier 21462
SMILES CC[N+](CC)(CC)CC(=O)NC1=C(C=CC=C1C)C.[Cl-]
Source Synthetic
InChi InChI=1S/C16H26N2O.ClH/c1-6-18(7-2,8-3)12-15(19)17-16-13(4)10-9-11-14(16)5;/h9-11H,6-8,12H2,1-5H3;1H
InChiKey LLPPOMUAOGMYQI-UHFFFAOYSA-N
MDL number MFCD01669894
Appearance White solid

References for QX 314 chloride

References are publications that support the biological activity of the product
  • Intracellular QX-314 inhibits calcium currents in hippocampal CA1 pyramidal neurons.

    Talbot MJ et al (1996) J Neurophysiol 76(3) : 2120-4.
  • Fast sodium action potentials are generated in the distal apical dendrites of rat hippocampal CA1 pyramidal cells.

    Colling SB et al (1994) Neurosci Lett 172(1-2) : 73-96.
Publications
These publications cite the use of QX 314 chloride purchased from Hello Bio:
  • Mild stress accumulation limits GABAergic synaptic plasticity in the lateral habenula

    Lalive AL et al (2022) Eur J Neurosci 55(2) : 377-387
    PubMedID: 34963191
  • Enhancement of parvalbumin interneuron-mediated neurotransmission in the retrosplenial cortex of adolescent mice following third trimester-equivalent ethanol exposure

    Bird CW et al (2021) Sci Rep 11(1) : 1716
    PubMedID: 33462326
  • Synaptic inhibition in the lateral habenula shapes reward anticipation

    Mameli et al (2021) bioRxiv . : https://doi.org/10.1101/2021.01.
  • Investigating dichotomous projections from ventral hippocampus to prefrontal cortex

    Sanchez Bellot et al (2020) UCL : Thesis
  • The Probability of Neurotransmitter Release Governs AMPA Receptor Trafficking via Activity-Dependent Regulation of mGluR1 Surface Expression.

    Sanderson et al (2018) Cell Rep. 25(13) : 3631-3646
    PubMedID: 30590038

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