Oxazole Yellow iodide is also known as Yo-Pro-1. It is a commonly used apoptosis marker.
Yo-Pro-1, YP1, YP-1
Oxazole Yellow iodide is also known as Yo-Pro-1 or YP1. It is a carbocyanine nucleic acid stain which has a strong binding affinity to nucleic acids.
It is a green ﬂuorescent DNA marker which is commonly used to identify apoptotic cells.
Uses and applications
Oxazole Yellow (YP1) does not penetrate the plasma membrane of viable cells. However, during apoptosis, apoptotic processes cause the cell-membrane to become slightly permeable. This allows Oxazole Yellow (YP1) to enter these cells and bind to nucleic acids to allow detection of apoptotic cells.
It is frequently used with propidium iodide when staining for apoptotic and necrotic cells as apoptotic cells remain impermeant to propidium iodide but permeable to Oxazole Yellow (YP1).
Oxazole yellow is a cyanine dye with strong affinity for nucleic acids used to stain apoptotic cells. Oxazole yellow from Hello Bio has an excitation peak at 491nm (when recorded at 507nm) and peak emission at 505nm (when excited at 490nm). For protocol see #Protocol 1 in application notes below.
#Protocol 1: Measurement of excitation and emission spectra of Oxazole Yellow
Oxazole yellow was prepared at 1pM in H20 with 33µg/ml empty plasmid DNA giving an approximate ratio of 1 dye molecule per 50bp of DNA.
Excitation and emission spectra were measured between 350nm and 700nm using a Tecan Infinite M200 PRO ELISA plate reader.
Excitation assessed using the emission wavelength of 507nm and emission was assessed using the excitation wavelength of 490nm.
Solubility & Handling
-20°C (protect from light)
Soluble in water (1 mg/ml), and in DMSO (1 mg/ml)
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use