Phorbol 12-myristate 13-acetate (PMA) product vial image | Hello Bio
Biological Data
Biological description
Phorbol 12-myristate 13-acetate (PMA) is a protein kinase C (PKC) activator which associates to the C1 domain. It is structurally analogous to diacylglycerol and has various biological actions. It induces ERK1/2 activation.
It is widely used to differentiate THP-1 cells for use as a model for function and biology of human macrophages.
It is frequently used with ionomycin to stimulate intracellular cytokine production in T-cells. Also used with Concanavalin A (ConA) to stimulate DNA and protein synthesis.
PMA also facilitates long term potentiation in hippocampal neurons via δ and ε PKC binding sites.
Additionally, PMA potentiates exocytosis and modulates vesicle fusion kinetics.
It is a potent tumor promoter.
Solubility & Handling
Solubility overview
Soluble in DMSO (100mM)
Storage instructions
-20°C (protect from light)
Storage of solutions
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
Phorbol ester phorbol-12-myristate-13-acetate promotes anchorage-independent growth and survival of melanomas through MEK-independent activation of ERK1/2.
Jørgensen K et al (2005) Biochem Biophys Res Commun 329(1) : 266-74.
![Phorbol 12-myristate 13-acetate [16561-29-8] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb0502.png "Phorbol 12-myristate 13-acetate [16561-29-8]")
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