Phorbol 12-myristate 13-acetate (PMA)

Technical documents: SDS CoA Datasheet

Product overview

Name Phorbol 12-myristate 13-acetate (PMA)
Alternative names PMA; TPA
Purity >99%
Customer comments

Shipping at RT but it works properly. cheap and quick delivery. Verified customer, Seoul National University

Description PKC activator. Widely used for THP-1 cell differentiation and with ionomycin for stimulating cytokine production
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Phorbol 12-myristate 13-acetate (PMA): Scientist Approved
Phorbol 12-myristate 13-acetate (PMA) product vial image | Hello Bio

Biological Data

Biological description

Phorbol 12-myristate 13-acetate (PMA) is a protein kinase C (PKC) activator which associates to the C1 domain. It is structurally analogous to diacylglycerol and has various biological actions. It induces ERK1/2 activation.

It is widely used to differentiate THP-1 cells for use as a model for function and biology of human macrophages.

It is frequently used with ionomycin to stimulate intracellular cytokine production in T-cells. Also used with Concanavalin A (ConA) to stimulate DNA and protein synthesis.

PMA also facilitates long term potentiation in hippocampal neurons via δ and ε PKC binding sites.

Additionally, PMA potentiates exocytosis and modulates vesicle fusion kinetics.

It is a potent tumor promoter.

Solubility & Handling

Storage instructions -20°C (protect from light)
Solubility overview Soluble in DMSO (100mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.



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Chemical Data

Purity >99%
Chemical name (1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-9a-(Acetyloxy)-1a,1b,4,4a,5,7a,7b,8,9,9a-decahydro-4a ,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz[1,2-e]azulen-9-yl tetradecanoate
Molecular Weight 616.83
Chemical structure Phorbol 12-myristate 13-acetate  [16561-29-8] Chemical Structure
Molecular Formula C36H56O8
CAS Number 16561-29-8
PubChem identifier 27924
SMILES CCCCCCCCCCCCCC(=O)O[C@@H]1[C@H]([C@]2([C@@H](C=C(C[C@]3([C@H]2C=C(C3=O)C)O)CO)[C@H]4[C@@]1(C4(C)C)OC(=O)C)O)C
InChi InChI=1S/C36H56O8/c1-7-8-9-10-11-12-13-14-15-16-17-18-29(39)43-32-24(3)35(42)27(30-33(5,6)36(30,32)44-25(4)38)20-26(22-37)21-34(41)28(35)19-23(2)31(34)40/h19-20,24,27-28,30,32,37,41-42H,7-18,21-22H2,1-6H3/t24-,27+,28-,30-,32-,34-,35-,36-/m1/s1
MDL number MFCD00036736

References for Phorbol 12-myristate 13-acetate (PMA)

References are publications that support the biological activity of the product
  • Phorbol 12-myristate 13-acetate inhibits P-glycoprotein-mediated efflux of DGX in MDCKII-MDR1 and Caco-2 cell monolayer models.

    Li YH et al (2014) Acta Pharmacol Sin 35(2) : 283-91.
  • Phorbol 12-Myristate 13-Acetate Enhances Long-Term Potentiation in the Hippocampus through Activation of Protein Kinase Cδ and ε.

    Kim EC et al (2013) Korean J Physiol Pharmacol 17(1) : 51-6.
  • Involvement of PKC alpha in PMA-induced facilitation of exocytosis and vesicle fusion in PC12 cells.

    Xue R et al (2009) Biochem Biophys Res Commun 380(2) : 371-6.
  • Phorbol ester phorbol-12-myristate-13-acetate promotes anchorage-independent growth and survival of melanomas through MEK-independent activation of ERK1/2.

    Jørgensen K et al (2005) Biochem Biophys Res Commun 329(1) : 266-74.

4 Item(s)

These publications cite the use of Phorbol 12-myristate 13-acetate (PMA) purchased from Hello Bio:
  • Ionic Mechanism Underlying Rebound Depolarization in Medial Prefrontal Cortex Pyramidal Neurons.

    Kurowski et al (2018) Front Cell Neurosci. : 12:93
    PubMedID: 29740284
  • mGluR-LTD at Excitatory and Inhibitory Synapses in the Lateral Habenula Tunes Neuronal Output.

    Valentinova and Mameli (2016) Cell Rep 16(9) : 2298-307
    PubMedID: 27545888

2 Item(s)