Figure 1. Memantine inhibition of evoked NMDAR mediated EPSCs in rat CA1 pyramidal neuron
The voltage sensitive NMDA receptor antagonist memantine is effective at concentrations of 10-100 µM. In CA1 hippocampal neurons held at -30 mV, memantine from Hello Bio (at 100 µM) gradually inhibited evoked NMDA receptor mediated excitatory currents over time. For assay protocol, see #Protocol 1 in Application Notes below.
Memantine hydrochloride: Scientist Approved
Memantine hydrochloride product vial image | Hello Bio
Figure 1. Memantine inhibition of evoked NMDAR mediated EPSCs in rat CA1 pyramidal neuron
The voltage sensitive NMDA receptor antagonist memantine is effective at concentrations of 10-100 µM. In CA1 hippocampal neurons held at -30 mV, memantine from Hello Bio (at 100 µM) gradually inhibited evoked NMDA receptor mediated excitatory currents over time. For assay protocol, see #Protocol 1 in Application Notes below.
Memantine hydrochloride: Scientist Approved
Memantine hydrochloride product vial image | Hello Bio
Biological Data
Biological description
Non-competitive NMDA receptor antagonist (IC50 = 1.25 μM). Binds to ion channel site.
Shows low affinity but has rapid blocking and unblocking ability at the NMDAR.
Selectively blocks extrasynaptic NMDARs.
Enhances hippocampal long-term potentiation (LTP) and reverses LTP suppression.
Improves cognitive function and shows anti-Alzheimer's activity.
Application notes
The voltage sensitive NMDA receptor antagonist memantine is effective at concentrations of 10-100 µM. In CA1 hippocampal neurons held at – 30 Mv, Hello Bio memantine (at 100 µM) gradually inhibited evoked NMDA receptor mediated excitatory currents over time (see Fig 1 above).
#Protocol 1: Assay evoked NMDAR currents at -30 mV (used for memantine)
NMDAR currents were recorded via whole cell voltage clamp recordings of CA1 pyramidal neurons from the rat hippocampal brain slice and evoked via a stimulating electrode placed in the CA3 region to stimulate the Schaffer collateral pathway.
Each NMDAR current was evoked via a single square (150 µs) pulse every 10 sec at a stimulus intensity that gave a reliable NMDAR current.
Neurons were constantly held at -30 mV and NMDAR currents recorded in response to continual bath applications of NMDAR antagonists.
All NMDAR recordings were made in the presence of GABAA-R and AMPA-R antagonists.
Solubility & Handling
Storage instructions
Room temperature
Solubility overview
Soluble in water (100mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
The N-methyl-D-aspartate receptor channel blockers memantine, MRZ 2/579 and other amino-alkyl-cyclohexanes antagonise 5-HT(3) receptor currents in cultured HEK-293 and N1E-115 cell systems in a non-competitive manner.
Rammes G et al (2001) Neurosci Lett 306(1-2) : 81-4.
![Memantine hydrochloride [41100-52-1] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb0407.png "Memantine hydrochloride [41100-52-1]")
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