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H2DCFDA

(HB8322)
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Product overview

Name H2DCFDA
Biological description Cell-permeable, fluorescent dye for ROS (reactive oxygen species e.g. H202) and NO (nitric oxide) detection. Commonly used for detection of ROS generation and to assess oxidative stress and redox status in cells and mitochondrial preparations. Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters suitable for fluorescein.
Purity >95%
Description Fluorescent dye for ROS and oxidative stress detection. Used to measure redox state of a cell.
Solubility & Handling Chemical Data Related Areas Calculators Technical guides (3) Reviews References (2) Frequently bought together
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Solubility & Handling

Storage instructions -20°C
Solubility overview Soluble in DMSO (100 mM), and in ethanol (10 mM)
Storage of solutions Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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More Info

Dilution

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More Info

Chemical Data

Purity >95%
Chemical name 2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid
Molecular Weight 485.27
Chemical structure H2DCFDA [4091-99-0] Chemical Structure
Molecular Formula

C24H14CI2O7

CAS Number 4091-99-0
PubChem identifier 77718
SMILES CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)O)Cl
InChiKey PXEZTIWVRVSYOK-UHFFFAOYSA-N
Excitation 485nm
Emission 535nm

References for H2DCFDA

References are publications that support the biological activity of the product

  • Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining.

    Kim H et al (2020) Journal of visualized experiments : JoVE :
    Read more (PubMedID: 32658187)

  • The involvement of TLR2 in cytokine and reactive oxygen species (ROS) production by PBMCs in response to Leishmania major phosphoglycans (PGs).

    Kavoosi G et al (2009) Parasitology 136 : 1193-9
    Read more (PubMedID: 19631014)

2 Item(s)

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Grouped product items
Order: HB8322
Pack PriceQty
100mg
$71
Fluorescent dye for ROS and oxidative stress detection. Used to measure redox state of a cell.
Shipping Info

Standard: $35

Hazardous: $35

Shipped on ice: $35

Shipping will be calculated at checkout

Contact Us

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Fax 609-228-4994

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