Propidium Iodide Staining Solution (1mg/ml) in water

Technical documents: SDS Datasheet

Product overview

Name Propidium Iodide Staining Solution (1mg/ml) in water
Description Red-fluorescent cell viability dye. 1mg/ml staining solution in water.
Alternative names PI
Biological description

Propidium iodide (PI) is a widely used red-fluorescent intercalating agent that binds and labels nucleic acids. This is a 1mg/ml staining solution.

Propidium iodide is membrane impermeant and is therefore frequently used to selectively identify dead cells and is commonly used in flow cytometry to evaluate cell viability.

Propidium iodide (PI) is often used in flow cytometry, fluorescent microscopy and confocal laser scanning microscopy applications.

Once bound to the nucleic acids, its fluorescence is enhanced 20- to 30-fold. Wavelength Maxima: Excitation ~535nm, Emission ~617nm

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Solubility & Handling

Storage instructions +4°C
Handling Protect from light.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use



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Chemical Data

Chemical name 2,7-Diamino-9-phenyl-10 (diethylaminopropyl)-phenanthridium iodide methiodide aqueous solution
Molecular Weight 668.39
Molecular Formula C27H34I2N4
CAS Number 25535-16-4
PubChem identifier 104981
SMILES CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-]
MDL number MFCD00011921
Appearance Red/pink solution
Excitation 535 nm
Emission 617 nm

References for Propidium Iodide Staining Solution (1mg/ml) in water

References are publications that support the biological activity of the product
  • The DNA intercalators ethidium bromide and propidium iodide also bind to core histones.

    Banerjee A et al (2014) FEBS Open Bio 4 : 251-9.
  • Analysis of apoptosis by propidium iodide staining and flow cytometry.

    Riccardi C et al (2006) Nat Protoc 1(3) : 1458-61.
  • DNA staining for fluorescence and laser confocal microscopy.

    Suzuki T et al (1997) J Histochem Cytochem 45(1) : 49-53.