Propidium Iodide Staining Solution (1mg/ml) in water
|Name|| Propidium Iodide Staining Solution (1mg/ml) in water |
|Description|| Red-fluorescent cell viability dye. 1mg/ml staining solution in water. |
|Biological description|| |
Propidium iodide (PI) is a widely used red-fluorescent intercalating agent that binds and labels nucleic acids. This is a 1mg/ml staining solution.
Propidium iodide is membrane impermeant and is therefore frequently used to selectively identify dead cells and is commonly used in flow cytometry to evaluate cell viability.
Propidium iodide (PI) is often used in flow cytometry, fluorescent microscopy and confocal laser scanning microscopy applications.
Once bound to the nucleic acids, its fluorescence is enhanced 20- to 30-fold. Wavelength Maxima: Excitation ~535nm, Emission ~617nm
Solubility & Handling
|Storage instructions|| +4°C |
|Handling|| Protect from light. |
|Important|| This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
|Chemical name|| 2,7-Diamino-9-phenyl-10 (diethylaminopropyl)-phenanthridium iodide methiodide aqueous solution |
|Molecular Formula|| C27H34I2N4 |
|PubChem identifier|| 104981 |
|SMILES|| CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-] |
|InChiKey|| XJMOSONTPMZWPB-UHFFFAOYSA-M |
|Appearance|| Red/pink solution |
References for Propidium Iodide Staining Solution (1mg/ml) in water
References are publications that support the biological activity of the product
The DNA intercalators ethidium bromide and propidium iodide also bind to core histones.
Banerjee A et al (2014) FEBS Open Bio 4 : 251-9.
Analysis of apoptosis by propidium iodide staining and flow cytometry.
Riccardi C et al (2006) Nat Protoc 1(3) : 1458-61.
DNA staining for fluorescence and laser confocal microscopy.
Suzuki T et al (1997) J Histochem Cytochem 45(1) : 49-53.