Propidium Iodide Staining Solution (1mg/ml) in water
(HB0821)
Product overview
| Name | Propidium Iodide Staining Solution (1mg/ml) in water |
| Alternative names | PI |
| Biological description | Propidium iodide (PI) is a widely used red-fluorescent intercalating agent that binds and labels nucleic acids. This is a 1mg/ml staining solution.
Frequently used with Oxazole Yellow (YP1) when staining for apoptotic and necrotic cells as apoptotic cells remain impermeant to propidium iodide but permeable to Oxazole Yellow (YP1). Â Once bound to the nucleic acids, its fluorescence is enhanced 20- to 30-fold. Wavelength Maxima: Excitation ~535nm, Emission ~617nm |
| Description | Red-fluorescent cell viability stain. 1mg/ml staining solution in water. |
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Images
Figure 1. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium lodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti- vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 2. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium iodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti-vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 3. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium iodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti-vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 4. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium iodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti-vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 1. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium lodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti- vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 2. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium iodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti-vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 3. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium iodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti-vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 4. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium iodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti-vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Biological Data
| Application notes | #Protocol 1: Propidium Iodide counterstaining of HEK293T cells
|
Solubility & Handling
| Storage instructions | +4°C |
| Handling | Protect from light. |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
Chemical Data
| Chemical name | 2,7-Diamino-9-phenyl-10 (diethylaminopropyl)-phenanthridium iodide methiodide aqueous solution |
| Molecular Weight | 668.39 |
| Molecular Formula | C27H34I2N4 |
| CAS Number | 25535-16-4 |
| PubChem identifier | 104981 |
| SMILES | CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-] |
| InChiKey | XJMOSONTPMZWPB-UHFFFAOYSA-M |
| MDL number | MFCD00011921 |
| Appearance | Red/pink solution |
| Excitation | 535 nm |
| Emission | 617 nm |
Technical guides
Understanding purity and quality - a guide for life scientistsReferences for Propidium Iodide Staining Solution (1mg/ml) in water
References are publications that support the biological activity of the product
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The DNA intercalators ethidium bromide and propidium iodide also bind to core histones.
Banerjee A et al (2014) FEBS Open Bio 4 : 251-9. -
Analysis of apoptosis by propidium iodide staining and flow cytometry.
Riccardi C et al (2006) Nat Protoc 1(3) : 1458-61. -
DNA staining for fluorescence and laser confocal microscopy.
Suzuki T et al (1997) J Histochem Cytochem 45(1) : 49-53.
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