DAPI Staining Solution (1mg/mL)

Figure 1: Neurofilament L and DAPI co-staining in hippocampal cell culture.

DAPI is a DNA binding dye commonly used to label cell nuclei in immunofluorescence experiments. DAPI from Hello Bio labels cell nuclei (blue) at 1µg/ml when co-stained with an anti-neurofilament L antibody (green). For protocol see #Protocol 1 in application notes below.

Figure 2: GFAP and DAPI co-staining in hippocampal cell culture.

DAPI is a DNA binding dye commonly used to label cell nuclei in immunofluorescence experiments. DAPI from Hello Bio labels cell nuclei (blue) at 1µg/ml when co-stained with an anti-GFAP antibody (green). For protocol see #Protocol 1 in application notes below.

Figure 3: MAP2 and DAPI co-staining in hippocampal cell culture.

DAPI is a DNA binding dye commonly used to label cell nuclei in immunofluorescence experiments. DAPI from Hello Bio labels cell nuclei (blue) at 1µg/ml when co-stained with an anti-MAP2 antibody (green). For protocol see #Protocol 1 in application notes below.

#Protocol 1: DAPI counterstaining of primary cultured neurones.

• Primary neurones were isolated and cultured from P2 rats and grown for three weeks before being fixed with 4% paraformaldehyde.
• Coverslips containing neuronal cell cultures were labelled for either MAP2, GFAP or Neurofilament L following standard immunohistochemical approaches.
• Coverslips were then submerged in 1µg/ml DAPI diluted in PBS for 1 minute.
• Following 2 x 5-minute washes in PBS coverslips were mounted and imaged with a fluorescent microscope.

Aliquot DAPI ready made solutions and store at -20°C and avoid freeze thaw cycles. This compound is light sensitive; exposure to light may affect compound performance, we therefore recommend storing solutions in the dark and protecting from light.