Product overview
Name | DAPI Staining Solution (1mg/mL) |
Description | Blue fluorescent DNA stain. Nuclear counterstain. 1mg/mL staining solution in water. Solid also available in 10mg and 50mg packs. |
Biological description | Overview DAPI is a blue fluorescent DNA stain which is cell permeant at high concentrations. This is a ready made 1mg/mL staining solution. It is recommended to dilute this solution 1:1000 with your desired buffer for a 1µg/mL working concentration. 0.1µg/mL-10µg/mL is the typical working concentration used in most applications. DAPI also available. |
Images
Biological Data
Application notes | Figure 1: Neurofilament L and DAPI co-staining in hippocampal cell culture. DAPI is a DNA binding dye commonly used to label cell nuclei in immunofluorescence experiments. DAPI from Hello Bio labels cell nuclei (blue) at 1µg/ml when co-stained with an anti-neurofilament L antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 2: GFAP and DAPI co-staining in hippocampal cell culture. DAPI is a DNA binding dye commonly used to label cell nuclei in immunofluorescence experiments. DAPI from Hello Bio labels cell nuclei (blue) at 1µg/ml when co-stained with an anti-GFAP antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 3: MAP2 and DAPI co-staining in hippocampal cell culture. DAPI is a DNA binding dye commonly used to label cell nuclei in immunofluorescence experiments. DAPI from Hello Bio labels cell nuclei (blue) at 1µg/ml when co-stained with an anti-MAP2 antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 4. Peak emission spectrum of oxazole yellow when excited at 360nm and excitation spectrum when recording at 460nm. DAPI is a DNA binding dye with strong affinity for nucleic acids used to label cell nuclei in immunofluorescence experiments. DAPI from Hello Bio has an excitation peak at 361nm and an emission peak at 456nm. For protocol see #Protocol 2 in application notes below.
#Protocol 1: DAPI counterstaining of primary cultured neurones.
#Protocol 2: Measurement of excitation and emission spectra of DAPI
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Solubility & Handling
Storage instructions | -20°C |
Handling | Aliquot DAPI ready made solutions and store at -20°C and avoid freeze thaw cycles. This compound is light sensitive; exposure to light may affect compound performance, we therefore recommend storing solutions in the dark and protecting from light. |
Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
Chemical Data
Chemical name | 4',6-Diamidino-2-phenylindole dihydrochloride |
CAS Number | 28718-90-3 |
Appearance | Pale yellow solution |
References for DAPI Staining Solution (1mg/mL)
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DAPI: a DNA-specific fluorescent probe.
Kapuscinski J (1995) Biotech Histochem 70(5) : 220-33. -
DAPI: a DNA-specific fluorescent probe.
Kapuscinski et al (1995) Biotech Histochem 70(5) : 220-33. -
DAPI as a useful stain for nuclear quantitation.
Tarnowski et al (1991) Biotech Histochem 66(6) : 297-302