Product overview
| Name | Oxazole Yellow Iodide 1mM solution (in 1mL DMSO) |
| Description | Oxazole Yellow iodide is also known as Yo-Pro-1. It is a commonly used apoptosis marker. 1mM solution (in 1mL DMSO) |
| Alternative names | Yo-Pro-1, YP1, YP-1 |
| Biological description | Overview Oxazole Yellow iodide is also known as Yo-Pro-1 or YP1. 1mM solution (in 1mL DMSO). It is a carbocyanine nucleic acid stain which has a strong binding affinity to nucleic acids. It is a green fluorescent DNA marker which is commonly used to identify apoptotic cells. Uses and applications Oxazole Yellow (YP1) does not penetrate the plasma membrane of viable cells. However, during apoptosis, apoptotic processes cause the cell-membrane to become slightly permeable. This allows Oxazole Yellow (YP1) to enter these cells and bind to nucleic acids to allow detection of apoptotic cells. It is frequently used with propidium iodide when staining for apoptotic and necrotic cells as apoptotic cells remain impermeant to propidium iodide but permeable to Oxazole Yellow (YP1). |
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Biological Data
| Application notes | Oxazole yellow is a cyanine dye with strong affinity for nucleic acids used to stain apoptotic cells. Oxazole yellow from Hello Bio has an excitation peak at 491nm (when recorded at 507nm) and peak emission at 505nm (when excited at 490nm). For protocol see #Protocol 1 in application notes below.
#Protocol 1: Measurement of excitation and emission spectra of Oxazole Yellow
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Solubility & Handling
| Storage instructions | -20°C (protect from light) |
| Solubility overview | DMSO solution |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
Chemical Data
| Chemical name | 4-[(3-Methyl-2(3H)-benzoxazolylidene)methyl]-1-[3-(trimethylammonio)propyl]-quinolinium diiodide |
| Molecular Weight | 629.32 |
| Molecular Formula | C24H29I2N3O |
| CAS Number | 152068-09-2 |
| PubChem identifier | 6439500 |
| SMILES | CN\1C2=CC=CC=C2O/C1=C\C3=CC=[N+](C4=CC=CC=C34)CCC[N+](C)(C)C.[I-].[I-] |
| Source | Synthetic |
| InChiKey | ULHRKLSNHXXJLO-UHFFFAOYSA-L |
| Appearance | Yellow Solution |
References for Oxazole Yellow Iodide 1mM solution (in 1mL DMSO)
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Evaluation of YO-PRO-1 as an early marker of apoptosis following radiofrequency ablation of colon cancer liver metastases
Fujisawa S et al (2014) Cytotechnology 66(2) : 259-73 -
Evaluation of YO-PRO-1 as an early marker of apoptosis following radiofrequency ablation of colon cancer liver metastases
Fujisawa S et al (2014) Cytotechnology 66(2) : 259-73 -
Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes
Wlodkowic D et al (2011) Methods Mol Biol 740 : 81-9 -
Application of the novel nucleic acid dyes YOYO-1, YO-PRO-1, and PicoGreen for flow cytometric analysis of marine prokaryotes
Marie D et al (1996) Appl Environ Microbiol 62(5) : 1649-55
Technical guides
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- Code:
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