Oxazole Yellow iodide is also known as Yo-Pro-1 or YP1. 1mM solution (in 1mL DMSO). It is a carbocyanine nucleic acid stain which has a strong binding affinity to nucleic acids.
It is a green fluorescent DNA marker which is commonly used to identify apoptotic cells.
Uses and applications
Oxazole Yellow (YP1) does not penetrate the plasma membrane of viable cells. However, during apoptosis, apoptotic processes cause the cell-membrane to become slightly permeable. This allows Oxazole Yellow (YP1) to enter these cells and bind to nucleic acids to allow detection of apoptotic cells.
It is frequently used with propidium iodide when staining for apoptotic and necrotic cells as apoptotic cells remain impermeant to propidium iodide but permeable to Oxazole Yellow (YP1).
Description
Oxazole Yellow iodide is also known as Yo-Pro-1. It is a commonly used apoptosis marker. 1mM solution (in 1mL DMSO)
Oxazole yellow is a cyanine dye with strong affinity for nucleic acids used to stain apoptotic cells. Oxazole yellow from Hello Bio has an excitation peak at 491nm (when recorded at 507nm) and peak emission at 505nm (when excited at 490nm). For protocol see #Protocol 1 in application notes below.
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Solubility & Handling
Storage instructions
-20°C (protect from light)
Solubility overview
DMSO solution
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
Understanding purity and quality - a guide for life scientists
