D-luciferin sodium salt is a water-soluble chemiluminescent luciferase substrate which is commonly used for in vivo imaging of the expression of luciferase. It is also frequently used in vitro.
The compound is nontoxic and stable in cells and live animals.
The luciferin substrate can be oxidized by the luciferase enzyme to generate an excited state molecule that emits light.
Dynamic bioluminescence imaging for quantitative tumour burden assessment using IV or IP administration of D: -luciferin: effect on intensity, time kinetics and repeatability of photon emission.
Keyaerts et al (2008) Eur J Nucl Med Mol Imaging. 35(5) : 999-1007