We would recommend Cmpd 101 from Hello Bio – it performs exactly as expected in assays looking at MOPr desensitisation, phosphorylation and internalisation. Dr Chris Bailey, University of Bath, UK and author on Mol Pharmacol paper, PubMed ID 26013542
Your Cmpd101 – worked great! Dr Steven Gee, Pfizer Neuroscience, USA
Your Cmpd101 behaved as expected.Verified customer, Monash University
How do I make a 30 uM concentration of Cmpd101 in aCSF, from an initial stock made in 100% DMSO?
If you prepare a stock solution of Cmpd101 dissolved in 100% DMSO to a concentration of 10-30 mM, you should then be able to take the final concentration down to 30 uM in ACSF (final DMSO 0.1%).
How do I solubilise (dissolve) Cmpd101?
You can solubilise Cmpd101 in DMSO, up to a concentration of 100 mM. Once Cmpd101 is in suspension, if you have problems solubilising it, you can try:
Stirring – try rapidly stirring or vortexing in a whirlimixer
Heating – try warming it gently in a water bath
Sonicating – sonication may also be worth a try
Temperature is very important when solubilising biochemicals. For example if you’ve cooled or frozen your Cmpd101 solution, it may have precipitated out of solution. So here’s an important rule – make sure you check that Cmpd101 is fully re-dissolved before use.
Do I need to re-weigh the Cmpd101 in my vial?
Yes - you should weigh out the quantity of Cmpd101 that you require for your experiment as the amount of product in our vials isn’t weighed out accurately enough for direct addition of solution. There are some exceptions to this but if this is the case, it will be stated clearly on the datasheet.
How do I store Cmpd101?
Storing Cmpd101 as a solid: If you keep the vial kept tightly sealed at room temperature, you can store Cmpd101 for up to 6months.
Storing and working with solutions of Cmpd101: If you are planning on storing and working with solutions of Cmpd101, we recommend preparing and using your solutions on the same day. However, if this isn’t possible and you need to prepare stock solutions of Cmpd101 beforehand, you should aliquot out the solution into tightly sealed vials at store at -20°C. We generally recommend that these will be useable for up to one month. You should also allow Cmpd101 to equilibrate to RT for at least one hour before opening and using.