Commonly used green fluorescent, intracellular pH indicator. Has a pKa of ~7, and exhibits pH-dependent, dual-excitation properties (Excitation 430/490nm, Emission 535nm) for ratiometric analysis. For HTS applications, BCECF can also be used in non-ratiometric mode using standard fluorescein excitation and emission settings. For optimal cell loading, F-127 is available either as a 10% solution in water (HB16503) and 20% solution in DMSO (HB9631).
This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
