Commonly used green fluorescent, intracellular pH indicator. Has a pKa of ~7, and exhibits pH-dependent, dual-excitation properties (Excitation 430/490nm, Emission 535nm) for ratiometric analysis. For HTS applications, BCECF can also be used in non-ratiometric mode using standard fluorescein excitation and emission settings. For optimal cell loading, F-127 is available either as a 10% solution in water (HB16503) and 20% solution in DMSO (HB9631).
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Handling
This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
