Product overview

Name BCECF AM
Alternative names 2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein, Acetoxymethyl Ester
Biological description

Commonly used green fluorescent, intracellular pH indicator. Has a pKa of ~7, and exhibits pH-dependent, dual-excitation properties (Excitation 430/490nm, Emission 535nm) for ratiometric analysis. For HTS applications, BCECF can also be used in non-ratiometric mode using standard fluorescein excitation and emission settings.

Purity >95%
Description

Green fluorescent membrane permeable pH indicator

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Solubility & Handling

Storage instructions -20°
Solubility overview

DMSO

Handling This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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Chemical Data

Purity >95%
Chemical name 3',6'-bis[(acetyloxy)methoxy]-5(or 6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoic acid, 2',7'-bis[(acetyloxy)methyl] ester
Molecular Weight 880.7
Chemical structure BCECF AM Structure Chemical Structure
Molecular Formula

C42H40O21

CAS Number 17464-70-7
PubChem identifier 145875767
SMILES O=C(C)OCOC1=C(CCC(OCOC(C)=O)=O)C=C(C2(C(C=CC(C(OCOC(C)=O)=O)=C3)=C3C(O2)=O)C(C(O4)=C5)=CC(CCC(OCOC(C)=O)=O)=C5OCOC(C)=O)C4=C1.O=C(C)OCOC6=C(CCC(OCOC(C)=O)=O)C=C(C7(C(C=C(C(OCOC(C)=O)=O)C=C8)=C8C(O7)=O)C(C(O9)=C%10)=CC(CCC(OCOC(C)=O)=O)=C%10OCOC(C)=O)C9=C6
InChiKey JBGGWLORWOIHKM-UHFFFAOYSA-N
Appearance Oil
Excitation 430, 490 nm
Emission 535 nm

FAQs

Can unused dye be frozen and reused?

Once dye has been dissolved in DMSO then it is possible to aliquot and freeze at -20°C. Try to avoid more than 2 freeze/thaw cycles.

References for BCECF AM

References are publications that support the biological activity of the product
  • A rapid method for measuring intracellular pH using BCECF-AM.

    Ozkan P et al (2002) Biochimica et biophysica acta 1572 : 143-8
  • Measurement of intracellular pH in cultured cells by flow cytometry with BCECF-AM.

    Franck P et al (1996) Journal of biotechnology 46 : 187-95
  • Use of fluorescent dye BCECF to measure intracellular pH in cortical collecting tubule.

    Weiner ID et al (1989) The American journal of physiology 256 : F957-64

3 Item(s)