Asante Potassium Green, Ion Potassium Green, APG, IPG, APG-2, IPG-2
Biological description
Yellow-green fluorescent potassium indicator (Kd = 18mM) which can be used with common filter sets (e.g. YFP and FITC) and multiphoton approaches (Excitation 525nm, Emission 545nm). Suitable for diverse applications such as extracellular K+ sensing and monitoring intracellular K+ dynamics. Synthetic fluorochrome which incorporates a K+-binding moiety. Under conditions where K+ is not bound, the fluorescence of the sensor is significantly quenched. When K+ is bound, the quenching is relieved, and the fluorescence of the sensor dramatically increases. Compatible with a wide variety of detectors including fluorescent microscopes, plate readers, flow cytometers, and fluorescent indicator-doped solid-state sensors.
Figure 1. Depletion of intracellular K+ through Na+/K+ ATPase inhibition measured using IPG-2
1mM Oubain and 50µM Gramicidin was applied to cells pre-incubated with NPG-2 following a 10 minute baseline. This leads to depletion of K+ which is observed as a decreased in IPG-2 fluorescence at 545nm compared to the control condition. For more details please see our IPG-2 protocol.
Figure 2. Concentration response of IPG-2 fluorescence in response to changing K+ concentration.
IPG-2 fluorescence at 545nm was measured in response to changing K+ concentration in 12.5mM Tris buffer pH7.4 containing 0.25% BSA, 1.2mM Mg2+ and TMA+ to maintain a constant ion concentration of 150mM. This resulted in a Kd of 18mM.
Figure 3. IPG-2 response to monovalent and divalent metal cations.
IPG-2 was screened against a panel of monovalent and divalent metal cations for increased fluorescence with ion concentration. Measurements were made in 140mM TMACI, 10mM Tris-HCl, 3µM IPG-2 for monovalent cations and 10mM EGTA, 10mM MOPS, 100mM K+, 3µM IPG-2, pH7.2 for divalent cations. Values are expressed relative to an ion-free reference solution.
Figure 1. Depletion of intracellular K+ through Na+/K+ ATPase inhibition measured using IPG-2
1mM Oubain and 50µM Gramicidin was applied to cells pre-incubated with NPG-2 following a 10 minute baseline. This leads to depletion of K+ which is observed as a decreased in IPG-2 fluorescence at 545nm compared to the control condition. For more details please see our IPG-2 protocol.
Figure 2. Concentration response of IPG-2 fluorescence in response to changing K+ concentration.
IPG-2 fluorescence at 545nm was measured in response to changing K+ concentration in 12.5mM Tris buffer pH7.4 containing 0.25% BSA, 1.2mM Mg2+ and TMA+ to maintain a constant ion concentration of 150mM. This resulted in a Kd of 18mM.
Figure 3. IPG-2 response to monovalent and divalent metal cations.
IPG-2 was screened against a panel of monovalent and divalent metal cations for increased fluorescence with ion concentration. Measurements were made in 140mM TMACI, 10mM Tris-HCl, 3µM IPG-2 for monovalent cations and 10mM EGTA, 10mM MOPS, 100mM K+, 3µM IPG-2, pH7.2 for divalent cations. Values are expressed relative to an ion-free reference solution.
This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
