Annexin V-FITC Apoptosis Staining / Detection Kit

(HB9623)
Technical documents: Datasheet

Product overview

Name Annexin V-FITC Apoptosis Staining / Detection Kit
Biological description

Identifies early apoptotic, necrotic and viable cells. One-step staining procedure which detects apoptosis by staining exposed phosphatidylserine (PS) which have translocated to the external cell surface. This allows detection of membrane changes that occur in early apoptotic cells. The kit is able to differentiate between:

 

  • Apoptotic cells: Stained by Annexin V-FITC but not propidium iodide
  • Necrotic cells: Stained by both Annexin V-FITC and propidium iodide
  • Live cells: Stained by neither Annexin V-FITC and propidium iodide

 

This kit contains:

  • Annexin V-FITC Staining solution
  • Propidium Iodide Staining solution
  • 10x binding buffer
Description Identifies early apoptotic, necrotic and viable cells
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Biological Data

Application notes

Please follow this link to a full Annexin V-FITC staining protocol.

Solubility & Handling

Storage instructions +4°C
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Excitation 491 nm
Emission 516 nm

References for Annexin V-FITC Apoptosis Staining / Detection Kit

References are publications that support the biological activity of the product
  • Past, present, and future of annexin A5: from protein discovery to clinical applications.

    Boersma HH et al (2005) Journal of nuclear medicine : official publication, Society of Nuclear Medicine 46 : 2035-50
  • A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V.

    Vermes I et al (1995) Journal of immunological methods 184 : 39-51

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