Ethidium homodimer I (EthD-I) is a membrane-impermeable, high-affinity, nucleic acid stain. Selectively labels dead cells with compromised plasma membranes. Compatible with flow cytometry, fluorescence microscopy, and plate reader assays.
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Handling
This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
Fluorescent labeling of DNA with ethidium homodimer without measurable decrease in DNA mobility: application to automated gel electrophoresis apparatus.
Zakharov SF et al (1995) Analytical biochemistry 224 : 195-8
