Product overview

Name Ethidium homodimer I
Alternative names Dead Cell Stain, EtDi, EthD-1
Biological description Ethidium homodimer I (EthD-I) is a membrane-impermeable, high-affinity, nucleic acid stain. Selectively labels dead cells with compromised plasma membranes. Compatible with flow cytometry, fluorescence microscopy, and plate reader assays.
Purity >90%
Description

Red fluorescent selective dead cell stain.

Write Your Own Review
You're reviewing:Ethidium homodimer I
Rate this item:

Solubility & Handling

Storage instructions -20°
Solubility overview

DMSO

Handling This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

=
x
x
More Info

Dilution

x
=
x
More Info

Chemical Data

Purity >90%
Chemical name 5-[3-[2-[3-(3,8-diamino-6-phenylphenanthridin-5-ium-5-yl)propylamino]ethylamino]propyl]-6-phenylphenanthridin-5-ium-3,8-diamine;dichloride;dihydrochloride
Molecular Weight 856.8
Chemical structure Ethidium Homodimer I Structure Chemical Structure
Molecular Formula

C46H50Cl4N8

CAS Number 61926-22-5
PubChem identifier 12328897
SMILES C1=CC=C(C=C1)C2=C3C=C(C=CC3=C4C=CC(=CC4=[N+]2CCCNCCNCCC[N+]5=C6C=C(C=CC6=C7C=CC(=CC7=C5C8=CC=CC=C8)N)N)N)N.Cl.Cl.[Cl-].[Cl-]
InChiKey ZKAJUKABUUJRLB-UHFFFAOYSA-N
Appearance Solid
Excitation 528 nm
Emission 618 nm

FAQs

Can unused dye be frozen and reused?

Once dye has been dissolved in DMSO then it is possible to aliquot and freeze at -20°C. Try to avoid more than 2 freeze/thaw cycles.

References for Ethidium homodimer I

References are publications that support the biological activity of the product
  • A novel method for the evaluation of proximal tubule epithelial cellular necrosis in the intact rat kidney using ethidium homodimer.

    Edwards JR et al (2007) BMC physiology 7 : 1
  • Fluorescent labeling of DNA with ethidium homodimer without measurable decrease in DNA mobility: application to automated gel electrophoresis apparatus.

    Zakharov SF et al (1995) Analytical biochemistry 224 : 195-8
  • A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels.

    Glazer AN et al (1990) Proceedings of the National Academy of Sciences of the United States of America 87 : 3851-5

3 Item(s)