Overview Aphidicolin is a potent DNA replication inhibitor which is often used to achieve cell synchronization.
Mechanism
Aphidicolin is a potent and specific inhibitor of B-family DNA polymerases and binds at or near the nucleotide-binding site. It prevents DNA polymerase-α from binding dNTPs without blocking the activity of DNA polymerase β or δ.
Aphidicolin inhibits DNA replication and some forms of DNA repair. During cell culture, addition of aphidicolin induces cell cycle pause at the G1/S border. DNA synthesis stops in cells that have entered S-phase, while nondividing cells are unaffected.
Uses Aphidicolin acts synergistically with vincristine and doxorubicin. In addition to its anti-mitotic effects, it exhibits antibiotic and antiviral activities.
Solubility & Handling
Solubility overview
Soluble in DMSO (25 mM)
Storage instructions
+4°C
Handling
This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the material in the dark and protecting from light.
Do not store the material in solution; make up solutions and use immediately.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
![(+)-Aphidicolin [38966-21-1] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb3690.png "(+)-Aphidicolin [38966-21-1]")
Understanding purity and quality - a guide for life scientists
