Product overview
| Name | aCSF (ready to use packets) |
| Biological description | Artificial cerebrospinal fluid (aCSF) is widely a widely used buffer in electrophysiological experiments to sustain ex-vivo brain sections. Simply add each packet to 1L of dH2O, mix and bubble with carbogen to make 1L of aCSF. Â Key features:
 Contains (in mM): NaCl 124. Glucose 10, NaHCO3 24, KCl 3, NaH2PO4 1.25, CaCl2 2.5, MgSO4 1.3  |
| Description | Preformulated packets to make artificial cerebrospinal fluid (aCSF) for electrophysiology. |
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Images
Figure 1. Representative traces of different experiment types carried out in HB9200 aCSF
Representative traces from four different experiments to measure NMDA dependent plateau potentials, excitatory post synaptic potentials (EPSPs), inhibitory postsynaptic currents (IPSCs) and excitatory postsynaptic currents (EPSCs). Method: C57BL/6J mouse brain sections were prepared following standard protocols (Udakis et al., 2020). Pyramidal neurons in hippocampal CA1 were patched using a CsMeSO4 internal solution containing QX314 and stimulated via the stratum radiatum to excite Schaffer collateral synapses.
Plateau potentials: Cells were held using current clamp and adjusted to a resting voltage of -69mV. Cells were then stimulated with one pulse followed by 5 stimuli at 100Hz frequency.
EPSP: Cells were held using current clamp and adjusted to a resting voltage of -69mV before being stimulated with a single pulse.
IPSC: Cells were held at 0mV using voltage clamp before being stimulated with a single pulse.
EPSC: Cells were held at -70mV before being stimulated via a paired pulse protocol with an interval of 50ms.
Plateau potentials: Cells were held using current clamp and adjusted to a resting voltage of -69mV. Cells were then stimulated with one pulse followed by 5 stimuli at 100Hz frequency.
EPSP: Cells were held using current clamp and adjusted to a resting voltage of -69mV before being stimulated with a single pulse.
IPSC: Cells were held at 0mV using voltage clamp before being stimulated with a single pulse.
EPSC: Cells were held at -70mV before being stimulated via a paired pulse protocol with an interval of 50ms.
Figure 2. Spontaneous EPSC and IPSC currents recorded using HB9200 aCSF
Traces showing both spontaneous excitatory postsynaptic currents (EPSCs) and spontaneous inhibitory postsynaptic currents (IPSCs). Method: C57BL/6J mouse brain sections were prepared following standard protocols (Udakis et al., 2020). Pyramidal neurons in hippocampal CA1 were patched using a CsMeSO4 internal solution containing QX314. Neurons were held in voltage clamp with sEPSCs being recorded at -70mV and sIPSCs being recorded at 0mV.
Figure 3. Long term recording of EPSCs in HB9200 aCSF for over 70 minutes
Summary of experiment recording excitatory post synaptic currents (EPSCs) for over 75 minutes in mouse pyramidal neurons. Method: C57BL/6J mouse brain sections were prepared following standard protocols (Udakis et al., 2020). Pyramidal neurons in hippocampal CA1 were patched using a CsMeSO4 internal solution containing QX314, held at -70mV in voltage clamp and stimulated in a paired pulse stimulation protocol (50ms interval) via the stratum radiatum to excite Schaffer collateral synapses. Figures shown are representative traces from different timepoints during the experiment, the input current required to hold the cell at -70mV, the series resistance, the amplitude of the initial EPSC and the ratio of amplitudes of the two EPSCs (paired pulse ratio).
Figure 1. Representative traces of different experiment types carried out in HB9200 aCSF
Representative traces from four different experiments to measure NMDA dependent plateau potentials, excitatory post synaptic potentials (EPSPs), inhibitory postsynaptic currents (IPSCs) and excitatory postsynaptic currents (EPSCs). Method: C57BL/6J mouse brain sections were prepared following standard protocols (Udakis et al., 2020). Pyramidal neurons in hippocampal CA1 were patched using a CsMeSO4 internal solution containing QX314 and stimulated via the stratum radiatum to excite Schaffer collateral synapses.
Plateau potentials: Cells were held using current clamp and adjusted to a resting voltage of -69mV. Cells were then stimulated with one pulse followed by 5 stimuli at 100Hz frequency.
EPSP: Cells were held using current clamp and adjusted to a resting voltage of -69mV before being stimulated with a single pulse.
IPSC: Cells were held at 0mV using voltage clamp before being stimulated with a single pulse.
EPSC: Cells were held at -70mV before being stimulated via a paired pulse protocol with an interval of 50ms.
Plateau potentials: Cells were held using current clamp and adjusted to a resting voltage of -69mV. Cells were then stimulated with one pulse followed by 5 stimuli at 100Hz frequency.
EPSP: Cells were held using current clamp and adjusted to a resting voltage of -69mV before being stimulated with a single pulse.
IPSC: Cells were held at 0mV using voltage clamp before being stimulated with a single pulse.
EPSC: Cells were held at -70mV before being stimulated via a paired pulse protocol with an interval of 50ms.
Figure 2. Spontaneous EPSC and IPSC currents recorded using HB9200 aCSF
Traces showing both spontaneous excitatory postsynaptic currents (EPSCs) and spontaneous inhibitory postsynaptic currents (IPSCs). Method: C57BL/6J mouse brain sections were prepared following standard protocols (Udakis et al., 2020). Pyramidal neurons in hippocampal CA1 were patched using a CsMeSO4 internal solution containing QX314. Neurons were held in voltage clamp with sEPSCs being recorded at -70mV and sIPSCs being recorded at 0mV.
Figure 3. Long term recording of EPSCs in HB9200 aCSF for over 70 minutes
Summary of experiment recording excitatory post synaptic currents (EPSCs) for over 75 minutes in mouse pyramidal neurons. Method: C57BL/6J mouse brain sections were prepared following standard protocols (Udakis et al., 2020). Pyramidal neurons in hippocampal CA1 were patched using a CsMeSO4 internal solution containing QX314, held at -70mV in voltage clamp and stimulated in a paired pulse stimulation protocol (50ms interval) via the stratum radiatum to excite Schaffer collateral synapses. Figures shown are representative traces from different timepoints during the experiment, the input current required to hold the cell at -70mV, the series resistance, the amplitude of the initial EPSC and the ratio of amplitudes of the two EPSCs (paired pulse ratio).
Solubility & Handling
| Storage instructions | RT. Add packet contents to 1L dH2O |
| Handling | Add the contents of each packet to 1000ml of dionised water and mix well. Bubble with carbogen and warm to 37°C before use. |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
Chemical Data
| Kit contents | Preformulated packets each making 1L of aCSF |
References for aCSF (ready to use packets)
References are publications that support the biological activity of the product
-
Reduced expression of the psychiatric risk gene DLG2 (PSD93) impairs hippocampal synaptic integration and plasticity.
Griesius S et al (2022) Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 47 : 1367-1378 -
The development of synaptic plasticity induction rules and the requirement for postsynaptic spikes in rat hippocampal CA1 pyramidal neurones.
Buchanan KA et al (2007) The Journal of physiology 585 : 429-45
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