Artificial cerebrospinal fluid (aCSF) is widely a widely used buffer in electrophysiological experiments to sustain ex-vivo brain sections. Simply add each packet to 1L of dH2O, mix and bubble with carbogen to make 1L of aCSF.
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Key features:
Save time using preformulated individual aCSF packets - each packet dissolves in seconds and there's no need to add Mg2+ or Ca2+
More reproducible with each pack's highly accurate formulation - less error for better data.
Extensively validated in a range of patch clamp electrophysiology experiments.
Figure 1. Representative traces of different experiment types carried out in HB9200 aCSF
Representative traces from four different experiments to measure NMDA dependent plateau potentials, excitatory post synaptic potentials (EPSPs), inhibitory postsynaptic currents (IPSCs) and excitatory postsynaptic currents (EPSCs). Method: C57BL/6J mouse brain sections were prepared following standard protocols (Udakis et al., 2020). Pyramidal neurons in hippocampal CA1 were patched using a CsMeSO4 internal solution containing QX314 and stimulated via the stratum radiatum to excite Schaffer collateral synapses.
Plateau potentials: Cells were held using current clamp and adjusted to a resting voltage of -69mV. Cells were then stimulated with one pulse followed by 5 stimuli at 100Hz frequency.
EPSP: Cells were held using current clamp and adjusted to a resting voltage of -69mV before being stimulated with a single pulse.
IPSC: Cells were held at 0mV using voltage clamp before being stimulated with a single pulse.
EPSC: Cells were held at -70mV before being stimulated via a paired pulse protocol with an interval of 50ms.
Figure 2. Spontaneous EPSC and IPSC currents recorded using HB9200 aCSF
Traces showing both spontaneous excitatory postsynaptic currents (EPSCs) and spontaneous inhibitory postsynaptic currents (IPSCs). Method: C57BL/6J mouse brain sections were prepared following standard protocols (Udakis et al., 2020). Pyramidal neurons in hippocampal CA1 were patched using a CsMeSO4 internal solution containing QX314. Neurons were held in voltage clamp with sEPSCs being recorded at -70mV and sIPSCs being recorded at 0mV.
Figure 3. Long term recording of EPSCs in HB9200 aCSF for over 70 minutes
Summary of experiment recording excitatory post synaptic currents (EPSCs) for over 75 minutes in mouse pyramidal neurons. Method: C57BL/6J mouse brain sections were prepared following standard protocols (Udakis et al., 2020). Pyramidal neurons in hippocampal CA1 were patched using a CsMeSO4 internal solution containing QX314, held at -70mV in voltage clamp and stimulated in a paired pulse stimulation protocol (50ms interval) via the stratum radiatum to excite Schaffer collateral synapses. Figures shown are representative traces from different timepoints during the experiment, the input current required to hold the cell at -70mV, the series resistance, the amplitude of the initial EPSC and the ratio of amplitudes of the two EPSCs (paired pulse ratio).
Figure 1. Representative traces of different experiment types carried out in HB9200 aCSF
Representative traces from four different experiments to measure NMDA dependent plateau potentials, excitatory post synaptic potentials (EPSPs), inhibitory postsynaptic currents (IPSCs) and excitatory postsynaptic currents (EPSCs). Method: C57BL/6J mouse brain sections were prepared following standard protocols (Udakis et al., 2020). Pyramidal neurons in hippocampal CA1 were patched using a CsMeSO4 internal solution containing QX314 and stimulated via the stratum radiatum to excite Schaffer collateral synapses.
Plateau potentials: Cells were held using current clamp and adjusted to a resting voltage of -69mV. Cells were then stimulated with one pulse followed by 5 stimuli at 100Hz frequency.
EPSP: Cells were held using current clamp and adjusted to a resting voltage of -69mV before being stimulated with a single pulse.
IPSC: Cells were held at 0mV using voltage clamp before being stimulated with a single pulse.
EPSC: Cells were held at -70mV before being stimulated via a paired pulse protocol with an interval of 50ms.
Figure 2. Spontaneous EPSC and IPSC currents recorded using HB9200 aCSF
Traces showing both spontaneous excitatory postsynaptic currents (EPSCs) and spontaneous inhibitory postsynaptic currents (IPSCs). Method: C57BL/6J mouse brain sections were prepared following standard protocols (Udakis et al., 2020). Pyramidal neurons in hippocampal CA1 were patched using a CsMeSO4 internal solution containing QX314. Neurons were held in voltage clamp with sEPSCs being recorded at -70mV and sIPSCs being recorded at 0mV.
Figure 3. Long term recording of EPSCs in HB9200 aCSF for over 70 minutes
Summary of experiment recording excitatory post synaptic currents (EPSCs) for over 75 minutes in mouse pyramidal neurons. Method: C57BL/6J mouse brain sections were prepared following standard protocols (Udakis et al., 2020). Pyramidal neurons in hippocampal CA1 were patched using a CsMeSO4 internal solution containing QX314, held at -70mV in voltage clamp and stimulated in a paired pulse stimulation protocol (50ms interval) via the stratum radiatum to excite Schaffer collateral synapses. Figures shown are representative traces from different timepoints during the experiment, the input current required to hold the cell at -70mV, the series resistance, the amplitude of the initial EPSC and the ratio of amplitudes of the two EPSCs (paired pulse ratio).
Solubility & Handling
Storage instructions
RT. Add packet contents to 1L dH2O
Handling
Add the contents of each packet to 1000ml of dionised water and mix well. Bubble with carbogen and warm to 37°C before use.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use