Cell permeable, synthetic estrogen receptor ligand. Widely used in genome engineering.
CreER system:
De facto standard compound in drug-inducible manipulation of CreER recombinase. Allows external temporal control of Cre activity in vivo.
May also be used for TRAPing / in the TRAP / TRAP2 system (Targeted recombination in active populations).
CRISPR/Cas9 gene editing:
Activates an inactived Cas9 nuclease (rendered inactive by insertion of a 4-OHT dependent-intein) to reduce off-target CRISPR-mediated gene editing (once bound with 4-OHT, conditionally active Cas9s modify target genomic sites with ~25-fold higher specificity than wild-type Cas9).
Also allows tight, repeated on-off control of the nuclease activity of the ‘iCas’ Cas9 variant which shows high editing efficiency at multiple loci once bound with 4-OHT.
This compound is light sensitive; exposure to light may affect compound performance. You should therefore store the material in the dark and protect from light.
Storage of solutions
Do not store the material in solution; make up solutions and use immediately:
The compound has been shown to isomerise rapidly in solution in most solvents (particularly solvents with a low dielectric constant). You should therefore make up and use solutions immediately.
The isomerisation process can be precluded by storage of the compound at -25°C in the dark as a THF solution containing ca. 0.025% BHT. (Katzenellenbogen et al (1982) J. Org. Chem. 47 2387.)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
These publications cite the use of (Z)-4-Hydroxytamoxifen (Z-4-OHT) purchased from Hello Bio:
Transcranial Low-Intensity Focused Ultrasound Stimulation of the Visual Thalamus Produces Long-Term Depression of Thalamocortical Synapses in the Adult Visual Cortex.
Mesik L et al (2024) The Journal of neuroscience : the official journal of the Society for Neuroscience 44
Antibody to c-Fos - an immediate early gene used as a marker of neuronal activity. Part of the ValidAb™ range of highly validated, data-rich antibodies.
![(Z)-4-Hydroxytamoxifen [68047-06-3] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb2508.png "(Z)-4-Hydroxytamoxifen [68047-06-3]")
Understanding purity and quality - a guide for life scientists
