4-Hydroxytamoxifen ≥70% Z isomer (remainder primarily E-isomer)
Alternative names
4-OHT, Afimoxifene, H6278, 4-HT, 4-OH-TAM, OHT, TAM, 4-Hydroxytamoxifen, tamoxifen, z-4oht
Purity
>98%
Customer comments
Great product, works great. Our normal vendor was backordered for 2 months, got excellent customer service from Hello Bio. Product worked great & delivery was fast. But best of all the price was great and we saved enough to buy other needed laboratory essentials. Researcher, University of Texas, USA
Perfect (4-Hydroxytamoxifen ≥70% Z isomer). No problem or concern about the quality: we compared with Sigma one and it works the same, but less expensive, immediate availability and fast delivery..Researcher, University of Lyon, France
Cell permeable, synthetic estrogen receptor ligand. Active metabolite of tamoxifen. Widely used for inducible genome manipulation. ≥70% Z isomer (remainder primarily E-isomer).
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Cre-LoxP / CreER system:
De facto standard for use in the inducible Cre-LoxP system for manipulation of CreER/CreERT2 recombinase for genome/ genetic manipulation (e.g. gene deletion). 4-OHT allows external temporal control of Cre activity in vivo.
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TRAP / TRAP2
Used as part of the TRAP/TRAP2 systems (targeted recombination in active populations) (e.g. FosTRAP, ArcTRAP) to provide genetic access to neurons.
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CRISPR/Cas9 gene editing:
Activates an inactived Cas9 nuclease (rendered inactive by insertion of a 4-OHT dependent-intein) to reduce off-target CRISPR-mediated gene editing (once bound with 4-OHT, conditionally active Cas9s modify target genomic sites with ~25-fold higher specificity than wild-type Cas9).
Also allows tight, repeated on-off control of the nuclease activity of the ‘iCas’ Cas9 variant which shows high editing efficiency at multiple loci once bound with 4-OHT.
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Cancer:
Chemotherapeutic agent. Induces apoptosis through an ER-dependent mechanism and inhibits proliferation of multiple myeloma cells in vitro.
This compound is light sensitive; exposure to light may affect compound performance. You should therefore store the material in the dark and protect from light.
Storage of solutions
Do not store the material in solution; make up solutions and use immediately:
The compound has been shown to isomerise rapidly in solution in most solvents (particularly solvents with a low dielectric constant). You should therefore make up and use solutions immediately.
The isomerisation process can be precluded by storage of the compound at -25°C in the dark as a THF solution containing ca. 0.025% BHT. (Katzenellenbogen et al (1982) J. Org. Chem. 47 2387.)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
Antibody to c-Fos - an immediate early gene used as a marker of neuronal activity. Part of the ValidAb™ range of highly validated, data-rich antibodies.
![70% Z-4-Hydroxytamoxifen [68392-35-8] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb6040.png "70% Z-4-Hydroxytamoxifen [68392-35-8]")
Understanding purity and quality - a guide for life scientists
