Novel, selective Piezo1 channel activator which shows improved (or equivalent) reliability, efficacy and potency in functional assays compared to Yoda1 (EC50 values are 150nM and 600nM at mouse Piezo1 in calcium assays for Yoda2 (KC289) and Yoda1 respectively). Selective for Piezo1 over other membrane proteins and suggested to have greater effect and potency at mouse Piezo1 compared to human Piezo1. Also shows improved aqueous solubility more suited to physiological conditions than those of Yoda1 (~160x more soluble in aqueous buffer than Yoda1). Active in vivo and shows vasorelaxant effects consistent with Piezo1 agonism. Recently shown to help promote cortical bone parameters in mice.
Solubility & Handling
Storage instructions
Room temperature
Solubility overview
Soluble in DMSO (50 mM with warming)
Handling
When Parsonage et al used Yoda2 (KC289) in universal aqueous buffer, a final DMSO concentration of 0.5% was used to maintain solubility of the compound.
Meslier et al prepared working solutions in 0.9% (w/v) Sodium Chloride, 5% DMSO, and 10% (w/v) cyclodextrin SBE-B-CD, to facilitate solubilization of the compound.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
Understanding purity and quality - a guide for life scientists
