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10x Western Blot Stripping Buffer

(HB9167)
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Product overview

Name 10x Western Blot Stripping Buffer
Biological description

Hello Bio 10x Western blot Stripping buffer allows the effective removal of primary and secondary antibodies from a PVDF or nitrocellulose membrane in order to facilitate subsequent reprobing of chemiluminescent Western blots. It protects membrane bound proteins and is quick and effective while not requiring use of a fume hood or elevated temperatures. 

Description

10x buffer for stripping of chemiluminescent Western blots

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Images

Figure 1. Stripping and re-probing of PVDF membrane using HB9167

Figure 1. Stripping and re-probing of PVDF membrane using HB9167

An array of samples from different tissues were loaded onto an acrylamide gel before being transferred to PVDF. The membrane was probed with HB0814 before being stripped with 1:10 diluted HB9167 following the protocol detailed in the application notes. The membrane was then successfully re-probed with HB9177. For more information on Western Blotting, please see our comprehensive Western blotting protocol
Figure 1. Stripping and re-probing of PVDF membrane using HB9167

Figure 1. Stripping and re-probing of PVDF membrane using HB9167

An array of samples from different tissues were loaded onto an acrylamide gel before being transferred to PVDF. The membrane was probed with HB0814 before being stripped with 1:10 diluted HB9167 following the protocol detailed in the application notes. The membrane was then successfully re-probed with HB9177. For more information on Western Blotting, please see our comprehensive Western blotting protocol

Biological Data

Application notes

Stripping Protocol

 

  1. Wash any remaining ECL solution off the membrane with a quick wash in PBS
  2. Freshly dilute 10x buffer down to 1x with dH2O
  3. Incubate twice for 10 minutes each in fresh stripping buffer
  4. Wash membrane briefly in two changes of PBST (PBS with 0.1% Tween20)
  5. Wash membrane 3 times in PBST for 5 minutes per wash
  6. Block memrane before proceeding with reprobing.

 

For any more details on Western Blotting techniques please see our Western blotting protocol.

Solubility & Handling

Storage instructions

Room temperature. Do not refrigerate.

Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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x
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Grouped product items
Order: HB9167
Pack PriceQty
500mL
$271
2x500mL
$406

10x buffer for stripping of chemiluminescent Western blots

Shipping Info

Standard: $35

Hazardous: $35

Shipped on ice: $35

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