Hello Bio 10x Western blot Stripping buffer allows the effective removal of primary and secondary antibodies from a PVDF or nitrocellulose membrane in order to facilitate subsequent reprobing of chemiluminescent Western blots. It protects membrane bound proteins and is quick and effective while not requiring use of a fume hood or elevated temperatures.Â
Description
10x buffer for stripping of chemiluminescent Western blots
Figure 1. Stripping and re-probing of PVDF membrane using HB9167
An array of samples from different tissues were loaded onto an acrylamide gel before being transferred to PVDF. The membrane was probed with HB0814 before being stripped with 1:10 diluted HB9167 following the protocol detailed in the application notes. The membrane was then successfully re-probed with HB9177. For more information on Western Blotting, please see our comprehensive Western blotting protocol
Figure 1. Stripping and re-probing of PVDF membrane using HB9167
An array of samples from different tissues were loaded onto an acrylamide gel before being transferred to PVDF. The membrane was probed with HB0814 before being stripped with 1:10 diluted HB9167 following the protocol detailed in the application notes. The membrane was then successfully re-probed with HB9177. For more information on Western Blotting, please see our comprehensive Western blotting protocol
Biological Data
Application notes
Stripping Protocol
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Wash any remaining ECL solution off the membrane with a quick wash in PBS
Freshly dilute 10x buffer down to 1x with dH2O
Incubate twice for 10 minutes each in fresh stripping buffer
Wash membrane briefly in two changes of PBST (PBS with 0.1% Tween20)
Wash membrane 3 times in PBST for 5 minutes per wash