Low-Cost Protease Inhibitor Cocktails & Phosphatase Inhibitor Cocktails
You can now protect your protein samples from degradation and preserve their phosphorylation state with our high quality, low-cost protease and phosphatase inhibitor cocktails - they are around half the price of other suppliers!
Our affordable low-cost range includes:
- Protease inhibitor cocktails,
- Phosphatase inhibitor cocktails, and
- Protease & phosphatase inhibitor cocktails.
Cell lysates contain endogenous proteases and phosphatases which degrade and modify proteins and these can adversely affect the sensitivity and reliability of your protein expression research. To protect your protein samples during cell lysis, protein extraction procedures and protein research (eg. Western blotting, reporter gene analysis, immunoprecipitations, epitope tagging, and specific protein activity assays), it is usual to add protease and/or phosphorylation inhibitors to your lysate samples.
Our low-cost protease and phosphatase inhibitor cocktails have been designed and specifically optimised to help protect the integrity of your proteins during cell lysis, extraction and purification, for a variety of tissues, enabling you to efficiently extract protein, preserve protein function and prevent protein degradation.
Protease Inhibitor Cocktails
Proteases are involved in many cellular functions, including cellular repair and the digestion of extracellular material. There are four main classes of proteolytic enzymes: serine proteases (eg. trypsin, chymotrypsin and elastase), cysteine proteases (eg. papain, calpain and lysosomal cathepsins), aspartic proteases ( eg.pepsin and rennin) and metalloproteases (eg.thermolysin and carboxypeptidase A).
As a broad guide:
- Serine proteases are widely distributed in most cell types,
- Bacterial extracts typically contain serine and metalloproteases
- Animal tissue extracts contain mainly serine, cysteine and metalloproteases (and some also contain aspartic proteases)
- Plant extracts contain large amounts of serine and cysteine proteases
Our protease inhibitor cocktails inhibit a number of proteases including serine, cysteine and aspartic proteases, and are suitable for animal and plant tissue extracts:
| Catalog number | Name | Optimized for use | Formulation | Targets | Components |
|---|---|---|---|---|---|
| HB9081 | Protease Inhibitor Cocktail solution (mammalian cell and tissue extract use (EDTA free) | Mammalian cell and tissue extract use | 1ml DMSO solution | aspartic, cysteine, serine proteases aminopeptidases |
AEBSF, Aprotinin, Bestatin, E-64, Leupeptin, Pepstatin A |
| HB9087 | Protease Inhibitor Cocktail solution (plant cell & tissue extract use) | Plant cell & tissue extract use | Solution | aspartic, cysteine, serine, metalloproteases aminopeptidases |
AEBSF, Bestatin, E-64, Leupeptin, Phenanthroline, Pepstatin A |
Phosphatase Inhibitor Cocktails
Phosphatases play a crucial role in regulating signal transduction. Protein kinases transfer a phosphate from ATP to a serine, threonine or tyrosine residue in a protein; phosphatases reverse this phosphorylation by removing the phosphoryl group. To preserve the phosphorylation state of the proteins in your samples, we offer a range of phosphatase inhibitor cocktails:
| Catalog number | Name | Optimized for use | Formulation | Targets | Components |
|---|---|---|---|---|---|
| HB9096 | Phosphatase Inhibitor Cocktail solution (cell lysates and tissue extract use incl. samples containing detergents) | Cell lysates and tissue extract use including samples containing detergents | 1ml DMSO solution | Acid & alkaline phosphatases Protein tyrosine phosphatases |
Imidazole, Sodium Fluoride, Sodium Molybdate, Sodium Orthovanadate, and Sodium Tartrate Dihydrate |
| HB9097 | Phosphatase Inhibitor Cocktail powder (cell lysates and tissue extract use incl. samples containing detergents) | Cell lysates and tissue extract use including samples containing detergents | Lyophilized powder | Acid & alkaline phosphatases Protein tyrosine phosphatases |
Imidazole, Sodium Fluoride, Sodium Molybdate, Sodium Orthovanadate, and Sodium Tartrate Dihydrate |
Protease and Phosphatase Inhibitor Cocktails
We also offer combined protease and phosphatase inhibitor cocktails, with and without EDTA, a metalloproteinase inhibitor:
| Catalog number | Name | Optimized for use | Formulation | Targets | Components |
|---|---|---|---|---|---|
| HB9104 | Protease & Phosphatase Inhibitor Cocktail solution (plus EDTA) | For both protease and phosphatases | 1ml H2O solution (plus EDTA) | serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases, metalloproteases | serine/threonine phosphatases, acid phosphatases, protein tyrosine phosphatases, alkaline phosphatases |
aprotinin (bovine lung), bestatin, E-64, leupeptin hemisulfate, β-glycerophosphate, sodium fluoride, sodium orthovanadate, sodium pyrophosphate decahydrate, EDTA disodium salt |
| HB9105 | Protease & Phosphatase Inhibitor Cocktail solution (EDTA free) | For both protease and phosphatases | 1ml H2O solution | serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases | serine/threonine phosphatases, acid phosphatases, protein tyrosine phosphatases, alkaline phosphatases |
aprotinin (bovine lung), bestatin, E-64, leupeptin hemisulfate, β-glycerophosphate, sodium fluoride, sodium orthovanadate, sodium pyrophosphate decahydrate |
Protease and phosphatase inhibitors available individually
- Leupeptin hemisulfate, a serine/cysteine protease inhibitor
- Pepstatin A, an aspartic protease inhibitor
- Aprotinin, a competitive serine protease inhibitor
- View all protease inhibitors
- View all phosphatase inhibitors
