Phosphatase Inhibitor Cocktail solution (cell lysates and tissue extract use incl. samples containing detergents)

(HB9096)
Technical documents: SDS Datasheet

Product overview

Name Phosphatase Inhibitor Cocktail solution (cell lysates and tissue extract use incl. samples containing detergents)
Description Phosphatase Inhibitor Cocktail solution for use in cell lysates and tissue extracts including samples containing detergents.
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Biological Data

Biological description

Overview

This protease inhibitor cocktail contains a mixture of protease inhibitors with broad specificity for various proteases. It is optimized for use in cell lysates and tissue extracts including samples containing detergents.

Protease inhibitor cocktails protect proteins from degradation by endogenous proteases released during protein extraction and purification.

Components and action

The following components are included in this cocktail which target phosphatases including protein tyrosine phosphatases, acid and alkaline phosphatases..

  • Imidazole: Alkaline phosphatase inhibitor (200mM)
  • Sodium fluoride: acid phosphatase and serine/threonine phosphatase inhibitor (100mM)
  • Sodium Molybdate: acid phosphate inhibit (115mM)
  • Sodium orthovanadate: protein tyrosine phosphatase/ alkaline phosphatase inhibitor (100mM)
  • Sodium tartrate dihydrate: acid phosphatase inhibitor (400mM)

Formulation & usage recommendation

The cocktail is supplied as a 1ml solution in water. Dilute 1:100 just prior to use.

 

Solubility & Handling

Storage instructions -20°C
Solubility overview Soluble in Water, DMSO (supplied in 1ml DMSO)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Appearance Clear liquid