This protease inhibitor cocktail contains a mixture of protease inhibitors with broad specificity for various proteases. It is optimized for use in cell lysates and tissue extracts including samples containing detergents.
Protease inhibitor cocktails protect proteins from degradation by endogenous proteases released during protein extraction and purification.
Components and action
The following components are included in this cocktail which target phosphatases including protein tyrosine phosphatases, acid and alkaline phosphatases.
Imidazole: Alkaline phosphatase inhibitor (200mM)
Sodium fluoride: acid phosphatase and serine/threonine phosphatase inhibitor (100mM)
Sodium Molybdate: acid phosphate inhibit (115mM)
Sodium orthovanadate: protein tyrosine phosphatase/ alkaline phosphatase inhibitor (100mM)
Reconstitute each vial with 1 ml H₂O. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Dilute 1:100 just prior to use.
Storage instructions
-20°C
Storage of solutions
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
Understanding purity and quality - a guide for life scientists
