Toxin produced by Bordetella pertussis . Catalyzes ADP-ribosylation of the α subunits of the heterotrimeric Gi/o proteins; Gαi, Gαo and Gαt . Blocks receptor coupling and activation by preventing G protein heterotrimers from interacting with receptors.
This product is not activated. Cells will activate pertussis toxin in an intact system however activation is required in a cell free system. Incubation with high concentrations of dithiothreitol (DTT) can achieve activation, see Kaslow, et al. (1987) for suggested conditions.
Solubility & Handling
Storage instructions
+4°C for lyophilised or resuspended (do not freeze)
Handling
Use solutions within 1 month, long term storage is not recommended.
Before use the suspension should be gently mixed (not vortexed) to make the suspension uniform. Do not sterile filter.
Pertussis toxin can be inactivated by boiling for 30mins
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
Y-27632 is a selective ROCK inhibitor. Frequently used 3D growth matrix component and for production of organoids. Also used when inducing neurons from fibroblasts. Manufactured to a high degree of optical purity.
Understanding purity and quality - a guide for life scientists
