Alkylation reagent. Commonly used to tag substrates for isolation by immunoprecipitation or immunoaffinity purification or detection by immunoblotting. Alkylates thiophosphates (e.g. substrates phosphorylated with ATPgammaS or N6-benzyl-ATPgammaS) to form thiophosphate ester epitopes that can be recognized by antibodies. Prepare a 50 mM PNBM (12 mg/ml) stock by dissolving PNBM in DMSO and vortexing.
Solubility & Handling
Storage instructions
-20°C
Solubility overview
soluble in DMSO to 100 mM
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
Y-27632 is a selective ROCK inhibitor. Frequently used 3D growth matrix component and for production of organoids. Also used when inducing neurons from fibroblasts. Manufactured to a high degree of optical purity.
Understanding purity and quality - a guide for life scientists
