Alkylation reagent. Commonly used to tag substrates for isolation by immunoprecipitation or immunoaffinity purification or detection by immunoblotting. Alkylates thiophosphates (e.g. substrates phosphorylated with ATPgammaS or N6-benzyl-ATPgammaS) to form thiophosphate ester epitopes that can be recognized by antibodies. Prepare a 50 mM PNBM (12 mg/ml) stock by dissolving PNBM in DMSO and vortexing.
Y-27632 is a selective ROCK inhibitor. Frequently used 3D growth matrix component and for production of organoids. Also used when inducing neurons from fibroblasts. Manufactured to a high degree of optical purity.