MTT is a tetrazolium dye which is commonly used in cell proliferation or cell growth assays In living cells, MTT is converted by NAD(P)H-dependent cellular oxidoreductase enzymes to its insoluble formazan which is purple/dark blue.
Uses and Applications
The color intensity can be measured colormetrically (at 540 or 570 nM) which allows quantitation of cell viability and cell proliferation.
Alternative names
Thiazolyl blue tetrazolium bromide
Purity
>97%
Description
Dye commonly used for cell proliferation measurement
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
Limitations of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay when compared to three commonly used cell enumeration assays
![MTT [298-93-1] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb5283.png "MTT [298-93-1]")
Understanding purity and quality - a guide for life scientists
