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X-Gal

(HB4128)
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Product overview

Name X-Gal
Alternative names Bromochloroindoxyl galactoside
Purity >99%
Description Detects presence and activity of β -galactosidase. Widely used in cloning applications.
Biological Data Solubility & Handling Chemical Data Related Areas Calculators Technical guides (1) Reviews References (4) Frequently bought together
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Biological Data

Biological description

Overview

X-Gal is a cell permeable, chromogenic substrate for β-galactosidase. It consits of galactose linked to a substituted indole.

β-galactosidase hydrolyzes X-gal to release an insoluble, bright blue precipitate.

Uses

X-Gal is frequently used in molecular biology cloning applications to detect the activity of β-galactosidase.

It is often used with IPTG in blue/white screening for bacterial colony selection in which X-Gal provides a visual indication of the cellular expression of β-galactosidase.

Solubility & Handling

Storage instructions +4°C
Solubility overview Soluble in DMSO (30 mg/ml)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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x
x
More Info

Dilution

x
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x
More Info

Chemical Data

Purity >99%
Chemical name 5-Bromo-4-chloro-3-(beta-D-galactopyranosyloxy)indole
Molecular Weight 408.6
Chemical structure X-Gal [7240-90-6] Chemical Structure
Molecular Formula C14H15BrClNO6
CAS Number 7240-90-6
PubChem identifier 65181
SMILES ClC1=C(C(O[C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O3)=CN2)C2=CC=C1Br
InChi InChI=1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
InChiKey OPIFSICVWOWJMJ-LMNFNBGQSA-N
MDL number MFCD00005666
Appearance White to off-white powder. Clear, colourless to light yellow in solution

References for X-Gal

References are publications that support the biological activity of the product

  • Overview and assessment of the histochemical methods and reagents for the detection of β-galactosidase activity in transgenic animals

    Trifonov S et al (2016) Anat Sci Int 91(1) : 56-67
    Read more (PubMedID: 26394634)

  • LacZ β-galactosidase: structure and function of an enzyme of historical and molecular biological importance

    Juers DH et al (2012) Protein Sci 21(12) : 1792-807
    Read more (PubMedID: 23011886)

  • Screening Bacterial Colonies Using X-gal and IPTG: α-Complementation

    Sambrook J et al (2006) CSH Protoc 2006 : 1
    Read more (PubMedID: 22485386)

  • Substrates For Cytochemical Demonstration Of Enzyme Activity. I. Some Substituted 3-Indolyl-Beta-D-Glycopyranosides

    Horwitz Jp Et Al (1964) J Med Chem 7 : 574-5
    Read more (PubMedID: 14221156)

4 Item(s)

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Grouped product items
Order: HB4128
Pack PriceQty
50mg
$29
250mg
$45
Detects presence and activity of β -galactosidase. Widely used in cloning applications.
Shipping Info

Standard: $35

Hazardous: $35

Shipped on ice: $35

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