Product overview

Name X-Gal
Alternative names Bromochloroindoxyl galactoside
Purity >99%
Description Detects presence and activity of β -galactosidase. Widely used in cloning applications.
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Biological Data

Biological description

Overview

X-Gal is a cell permeable, chromogenic substrate for β-galactosidase. It consits of galactose linked to a substituted indole.

β-galactosidase hydrolyzes X-gal to release an insoluble, bright blue precipitate.

Uses

X-Gal is frequently used in molecular biology cloning applications to detect the activity of β-galactosidase.

It is often used with IPTG in blue/white screening for bacterial colony selection in which X-Gal provides a visual indication of the cellular expression of β-galactosidase.

Solubility & Handling

Solubility overview Soluble in DMSO (30 mg/ml)
Storage instructions +4°C
Storage of solutions Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Purity >99%
Chemical name 5-Bromo-4-chloro-3-(beta-D-galactopyranosyloxy)indole
Molecular Weight 408.6
Chemical structure X-Gal [7240-90-6] Chemical Structure
Molecular Formula C14H15BrClNO6
CAS Number 7240-90-6
PubChem identifier 65181
SMILES ClC1=C(C(O[C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O3)=CN2)C2=CC=C1Br
InChi InChI=1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
InChiKey OPIFSICVWOWJMJ-LMNFNBGQSA-N
MDL number MFCD00005666
Appearance White to off-white powder. Clear, colourless to light yellow in solution

References for X-Gal

References are publications that support the biological activity of the product
  • Overview and assessment of the histochemical methods and reagents for the detection of β-galactosidase activity in transgenic animals

    Trifonov S et al (2016) Anat Sci Int 91(1) : 56-67
  • LacZ β-galactosidase: structure and function of an enzyme of historical and molecular biological importance

    Juers DH et al (2012) Protein Sci 21(12) : 1792-807
  • Screening Bacterial Colonies Using X-gal and IPTG: α-Complementation

    Sambrook J et al (2006) CSH Protoc 2006 : 1
  • Substrates For Cytochemical Demonstration Of Enzyme Activity. I. Some Substituted 3-Indolyl-Beta-D-Glycopyranosides

    Horwitz Jp Et Al (1964) J Med Chem 7 : 574-5

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