X-Gal is a cell permeable, chromogenic substrate for β-galactosidase. It consits of galactose linked to a substituted indole.
β-galactosidase hydrolyzes X-gal to release an insoluble, bright blue precipitate.
Uses
X-Gal is frequently used in molecular biology cloning applications to detect the activity of β-galactosidase.
It is often used with IPTG in blue/white screening for bacterial colony selection in which X-Gal provides a visual indication of the cellular expression of β-galactosidase.
Solubility & Handling
Solubility overview
Soluble in DMSO (30 mg/ml)
Storage instructions
+4°C
Storage of solutions
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
![X-Gal [7240-90-6] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb4128.png "X-Gal [7240-90-6]")
Understanding purity and quality - a guide for life scientists
